Lack of the Consensus Sequence Necessary for Tryptophan Prenylation in the ComX Pheromone Precursor
Lack of the Consensus Sequence Necessary for Tryptophan Prenylation in the ComX Pheromone Precursor
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DOI：
10.1271/bbb.120206
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发表时间：
2012-08-01
影响因子：
1.6
通讯作者：
Sakagami, Youji
中科院分区：
文献类型：
--
作者：
Tsuji, Fumitada;Ishihara, Ayako;Sakagami, Youji
ComX是一种寡肽信息素，能刺激枯草芽孢杆菌及其相关芽孢杆菌群体感应控制的天然遗传能力，含有异戊二烯基修饰的色氨酸残基。由于ComX是唯一已知含有异戊烯化色氨酸的蛋白质，这种独特的翻译后修饰的普遍性尚未确定。最近，我们开发了一种无细胞检测系统，其中ComX（RO-E-2）信息素前体中的色氨酸残基来自B。枯草芽孢杆菌菌株RO-E-2可以被ComQ（RO-E-2）酶香叶基化。我们在这里报告我们的尝试，以确定周围的香叶基色氨酸残基的共识序列，通过使用各种ComX（RO-E-2）信息素前体类似物的无细胞系统。我们发现[47-58]ComX（RO-E-2），对应于信息素前体的C-末端12-残基肽，含有香叶基化所必需的短序列。我们还发现，色氨酸残基和C-末端之间的序列的长度对于香叶基化是重要的，并且一些[47-58]ComX（RO-E-2）信息素前体氨基酸参与香叶基化反应。然而，我们不能确定围绕香叶基化色氨酸的共有序列。我们的证据表明，像拉布缺乏一个共识序列，但香叶基香叶基修饰的半胱氨酸残基，ComX信息素及其前体也缺乏一个共识序列。
ComX, an oligopeptide pheromone that stimulates the natural genetic competence controlled by quorum sensing in Bacillus subtilis and related bacilli, contains a prenyl-modified tryptophan residue. Since ComX is the only protein known to contain prenylated tryptophan, the universality of this unique posttranslational modification has yet to be determined. Recently, we developed a cell-free assay system in which the tryptophan residue in the ComX(RO-E-2) pheromone precursor derived from B. subtilis strain RO-E-2 can be geranylated by the ComQ(RO-E-2) enzyme. We report here our attempt to identify the consensus sequence surrounding the geranylated tryptophan residue by using the cell-free system with various ComX(RO-E-2) pheromone precursor analogs. We found that [47-58]ComX(RO-E-2), corresponding to the C-terminal 12-residue peptide of the pheromone precursor, contained a short sequence essential for geranylation. We also found that the length of the sequence between the tryptophan residue and the C-terminus was important for geranylation, and that some [47-58]ComX(RO-E-2) pheromone precursor amino acids were involved in the geranylation reaction. However, we could not identify a consensus sequence surrounding the geranylated tryptophan. Our evidence suggests that, like Rab which lacks a consensus sequence yet is geranylgeranyl-modified on a cysteine residue, the ComX pheromone and its precursor also lack a consensus sequence.