Boxcar detection for high-frequency modulation in stimulated Raman scattering microscopy
Boxcar detection for high-frequency modulation in stimulated Raman scattering microscopy
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DOI：
10.1063/1.5022266
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发表时间：
2018-04-16
影响因子：
4
通讯作者：
Zumbusch, A.
中科院分区：
文献类型：
--
作者：
Fimpel, P.;Riek, C.;Zumbusch, A.
受激拉曼散射（SRS）显微镜是一种重要的非线性光学技术，用于研究未标记样品。SRS信号本身表现为参与拉曼模式的相干激发的激光脉冲之间的小强度交换。通常，在一个脉冲串中应用高频调制，然后通过锁定放大在另一个脉冲串上检测信号。虽然允许散粒噪声限制的检测灵敏度，但是对应于在频域中过滤信号的锁定检测不是使用激发光的最有效的方式。在这篇手稿中，我们表明，boxcar平均，这是相当于时间滤波，更适合于检测低占空比信号中遇到的SRS显微镜。我们证明，通过采用合适的门控窗口，可以实现锁定检测的信噪比在较短的时间与boxcar平均。因此，以更快的速率和更低的辐照度记录高质量的图像，这是一个重要因素，例如，用于使生物样品的降解最小化。出版社：AIP Publishing
Stimulated Raman scattering (SRS) microscopy is an important non-linear optical technique for the investigation of unlabeled samples. The SRS signal manifests itself as a small intensity exchange between the laser pulses involved in coherent excitation of Raman modes. Usually, high-frequency modulation is applied in one pulse train, and the signal is then detected on the other pulse train via lock-in amplification. While allowing shot-noise limited detection sensitivity, lock-in detection, which corresponds to filtering the signal in the frequency domain, is not the most efficient way of using the excitation light. In this manuscript, we show that boxcar averaging, which is equivalent to temporal filtering, is better suited for the detection of low-duty-cycle signals as encountered in SRS microscopy. We demonstrate that by employing suitable gating windows, the signal-to-noise ratios achievable with lock-in detection can be realized in shorter time with boxcar averaging. Therefore, high-quality images are recorded at a faster rate and lower irradiance which is an important factor, e.g., for minimizing degradation of biological samples. Published by AIP Publishing.