Simultaneous production and recovery of fumaric acid from immobilized Rhizopus oryzae with a rotary biofilm contactor and an adsorption column
Simultaneous production and recovery of fumaric acid from immobilized Rhizopus oryzae with a rotary biofilm contactor and an adsorption column
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DOI：
10.1128/aem.62.8.2926-2931.1996
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发表时间：
1996-08-01
影响因子：
4.4
通讯作者：
Tsao, GT
中科院分区：
文献类型：
--
作者：
Cao, NJ;Du, JX;Tsao, GT
研究了米根霉从葡萄糖中生产和回收富马酸的同时发酵-吸附一体化系统，该系统在富氮生长阶段将生长的根霉菌丝自固定在旋转生物膜接触器的塑料圆盘上，在非生长生产阶段，生物膜在水平发酵容器中旋转圆盘交替暴露于液体介质和空气中。通过耦合吸附柱同时连续去除发酵产物富马酸，从而减轻了抑制，提高了发酵速率，维持了细胞活力。去除富马酸的另一个有益效果是聚乙烯醇吡啶吸附剂将羟基离子释放到循环发酵液中。聚乙烯吡啶和IRA-900为这种类型的发酵提供了最高的负载，这种发酵系统能够在重复的补料间歇循环下，在20小时内从每升100克葡萄糖中生产富马酸，平均产量为85克/升，在重量产率的基础上，以4.25克/升/小时的生产率获得理论最大值的91%。这与添加碳酸钙的搅拌池发酵形成对比，后者在72 h后的平均产重率为65%，生产率为0.9 g/l /h。固定化反应器重复运行2周，生物活性无损失。
An integrated system of simultaneous fermentation-adsorption for the production and recovery of fumaric acid from glucose by Rhizopus oryzae was investigated, The system was constructed such that growing Rhizopus mycelia were self-immobilized on the plastic discs of a rotary biofilm contactor during the nitrogen-rich growth phase, During the nongrowth, production phase, the biofilm was alternately exposed to liquid medium and air upon rotation of the discs in the horizontal fermentation vessel. The product of fermentation, fumaric acid, was removed simultaneously and continuously by a coupled adsorption column, thereby moderating inhibition, enhancing the fermentation rate, and sustaining cell viability, Another beneficial effect of the removal of fumaric acid is release of hydroxyl ions from a polyvinyl pyridine adsorbent into the circulating fermentation broth. This moderates the decrease in pH that would otherwise occur, Polyvinyl pyridine and IRA-900 gave the highest loading for this type of fermentation, This fermentation system is capable of producing fumaric acid with an average yield of 85 g/liter from 100 g of glucose per liter within 20 h under repetitive Fed-batch cycles, On a weight yield basis, 91% of the theoretical maximum was obtained with a productivity of 4.25 g/liter/h. This is in contrast to stirred-tank fermentation supplemented with calcium carbonate, whose average weight yield was 65% after 72 h with a productivity of 0.9 g/liter/h. The immobilized reactor was operated repetitively for 2 weeks without loss of biological activity.