A functional peptide-mediated colorimetric assay for mercury ion based on dual-modified gold nanoparticles
A functional peptide-mediated colorimetric assay for mercury ion based on dual-modified gold nanoparticles
复制标题
基于双修饰金纳米粒子的功能性肽介导的汞离子比色测定
DOI：
10.1016/j.ab.2021.114369
复制
发表时间：
2021-09-14
影响因子：
2.9
通讯作者：
Hu, Ji-Ming
中科院分区：
文献类型：
--
作者：
Li, Xin-Yi;Zhang, Miao-Miao;Hu, Ji-Ming
本工作构建了一种快速、准确的汞离子（Hg 2+）生物传感器，利用肽对Hg 2+的高度特异性，触发双修饰（DGPFHR-和CALNN-）金纳米粒子（D/C-AuNPs）的聚集。DGPFHR具有很强的捕获Hg ~（2+）的能力，并伴有构象折叠。在此基础上，采用D/C-AuNPs作为检测探针，实现了Hg 2+的定量分析。这主要是因为特定的Hg 2+诱导的肽折叠减少了静电排斥和空间位阻，从而加速了AuNPs的聚集。该方案的原理和应用潜力得到了实证证明。结果表明，Hg 2+离子在100-800 nM范围内线性关系良好，最低检测浓度为28 nM。该方法具有上级的简便性、选择性、准确性和稳定性，在实际水样的测定中优于其它方案。
In the work, a rapid and accurate biosensor for mercury ions (Hg2+) was constructed, with which aggregation of dual-modified (DGPFHR- and CALNN-) gold nanoparticles (D/C-AuNPs) could be triggered by the high specificity of peptides to Hg2+. The given peptide DGPFHR possesses great capability of capturing Hg2+, accompanied by the conformational folding. Under the circumstances, D/C-AuNPs were employed as the detection probes to accomplish the quantitative analysis of Hg2+. This is primarily because the specific Hg2+-induced folding of peptides reduces the electrostatic repulsion and steric hindrance, thus accelerating the AuNPs aggregation. The principle and application potential of this proposal was proved by evidence. And the results demonstrated that Hg2+ ions could be selectively detected as low as 28 nM with a linear range of 100-800 nM. In consideration of superior simplicity, selectivity, accuracy and stability, the protocol was advantageous over other projects in practical measurement of various water samples.