A technique for demonstrating the nerve supply of whole larynges.
A technique for demonstrating the nerve supply of whole larynges.
复制标题
展示整个喉部神经供应的技术。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
I. Sanders
中科院分区：
文献类型：
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作者：
B. Wu;I. Sanders
目前，我们对感觉神经和运动神经在喉内的确切分布的理解还有很大的差距。大体解剖仅对大口径神经准确，而显微镜切片仅显示孤立的终末分支。为了填补这一空白，我们修改了一种称为Sihler染色的旧技术，以处理五个完整的犬喉。这项技术需要八个步骤，大约需要三个月。结果是整个喉，其中肌肉和软骨呈现半透明，而神经被复染。从大口径神经到终末支可连续追踪神经分支。我们发现，神经模式是令人惊讶的复杂，并包含频繁的吻合网络。这项技术是研究某些神经喉学问题的有力工具。
At present there is a large gap in our understanding of the exact distribution of sensory and motor nerves within the larynx. Gross dissection is only accurate for large-caliber nerves, whereas microscopic sections show only isolated examples of terminal branching. To fill this gap, we modified an old technique called Sihler's stain to process five whole canine larynges. The technique requires eight steps that take approximately 3 months. The result is an entire larynx in which muscle and cartilage are rendered translucent, while nerve is counterstained. Nerve branching can be continuously traced from the large-caliber nerves to the terminal branches. We found that the nerve patterns are surprisingly complex and contain frequent anastomotic networks. This technique is a powerful tool for studying certain neurolaryngological problems.