FBF-1 and FBF-2 regulate the size of the mitotic region in the C-elegans germline
FBF-1 and FBF-2 regulate the size of the mitotic region in the C-elegans germline
复制标题
DOI：
10.1016/j.devcel.2004.09.013
复制
发表时间：
2004-11-01
影响因子：
11.8
通讯作者：
Kimble, J
中科院分区：
文献类型：
--
作者：
Lamont, LB;Crittenden, SL;Kimble, J
在秀丽隐杆线虫种系中，GLIP-1/Notch 信号传导和两种几乎相同的 RNA 结合蛋白 FBF-1 和 FBF-2 促进增殖。在这里，我们发现 fbf-1 和 fbf-2 基因在促进有丝分裂方面很大程度上是多余的，但它们在微调有丝分裂区域的大小方面具有相反的作用。 fbf-1 突变体的有丝分裂区域小于正常区域，但 fbf-2 突变体的有丝分裂区域大于正常区域。与基因特异性作用一致，fbf-2 表达仅限于远端种系，而 fbf-1 表达范围更广。 fbf-2 基因（但显然不是 fbf-1）受 GLP-1/Notch 信号传导控制，并且 FBF-1 和 FBF-2 蛋白的丰度受到相互 3'UTR 抑制的限制。我们认为不同的 fbf 基因及其调节子网络能够精确控制有丝分裂区域的大小。因此，fbf-1和fbf-2为最近复制的基因如何分化以微调动物发育过程中的模式提供了范例。
In the C. elegans germline, GLIP-1/Notch signaling and two nearly identical RNA binding proteins, FBF-1 and FBF-2, promote proliferation. Here, we show that the fbf-1 and fbf-2 genes are largely redundant for promoting mitosis but that they have opposite roles in fine-tuning the size of the mitotic region. The mitotic region is smaller than normal in fbf-1 mutants but larger than normal in fbf-2 mutants. Consistent with gene-specific roles, fbf-2 expression is limited to the distal germline, while fbf-1 expression is broader. The fbf-2 gene, but apparently not fbf-1, is controlled by GLP-1/Notch signaling, and the abundance of FBF-1 and FBF-2 proteins is limited by reciprocal 3'UTR repression. We propose that the divergent fbf genes and their regulatory subnetwork enable a precise control over size of the mitotic region. Therefore, fbf-1 and fbf-2 provide a paradigm for how recently duplicated genes can diverge to fine-tune patterning during animal development.