Application of MALDI-TOF MS for Estimating the Postmortem Interval in Rat Muscle Samples
Application of MALDI-TOF MS for Estimating the Postmortem Interval in Rat Muscle Samples
复制标题
应用 MALDI-TOF MS 估算大鼠肌肉样本的死后间隔
DOI：
10.1111/1556-4029.13413
复制
发表时间：
2017-09-01
影响因子：
1.6
通讯作者：
Wang, Zhenyuan
中科院分区：
文献类型：
--
作者：
Li, Chengzhi;Ma, Dong;Wang, Zhenyuan
死亡时间的推断在法医学中具有重要意义。虽然许多方法已被用于估计PMI，但准确的PMI计算仍然很困难。在这项研究中，四个Sprague道利（SD）大鼠通过窒息处死，并通过解剖收集肌肉样品在不同的时间间隔（0，48，96，和144小时）后死亡。使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOFMS）探测所有样品，以获得分子图像和主成分分析（PCA）数据。结果表明，m/z 1511、1543、1564、1586处的峰在死后0至144 h的强度明显降低，并且在PCA评分图上时间组彼此分离。预测模型具有较高的识别率（95.93%）和交叉验证率（83.72%）。我们的工作表明，MALDI-TOF MS可以用来确定PMI。
Estimating the postmortem interval (PMI) is very important in the forensic sciences. Although many approaches have been used for estimating the PMI, accurate PMI calculations are still difficult. In this study, four Sprague Dawley (SD) rats were sacrificed by suffocation, and muscle samples were collected by dissection at various time intervals (0, 48, 96, and 144 h) after death. All samples were probed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) to obtain molecular images and data for principal component analysis (PCA). The results showed that the peaks at m/z 1511, 1543, 1564, 1586 clearly decreased in intensity from 0 to 144 h postmortem and that the time groups were separated from each other on the PCA score plot. The prediction model showed high recognition capability (95.93%) and cross-validation (83.72%). Our work suggests that MALDI-TOF MS can be used to determine the PMI.