Formins control dynamics of F-actin in the central cell of Arabidopsis thaliana
Formins control dynamics of F-actin in the central cell of Arabidopsis thaliana
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DOI：
10.1080/15592324.2021.1920192
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发表时间：
2021-05-05
影响因子：
2.9
通讯作者：
Kawashima, Tomokazu
中科院分区：
文献类型：
--
作者：
Ali, Mohammad Foteh;Kawashima, Tomokazu
在开花植物的雌性配子中，精子核的迁移受肌动蛋白丝（F-actin）不断向内运动的控制，从而成功受精。这种动态的f -肌动蛋白运动是与arp2 /3无关的，这就提出了在雌性配子中肌动蛋白成核和聚合是如何被控制的问题。利用共聚焦显微镜活细胞成像结合药理学方法，我们评估了另一组肌动蛋白成核蛋白，形成蛋白，参与f -肌动蛋白向内运动在拟南芥的中心细胞。我们发现，formin抑制剂SMIFH2对formin功能的抑制显著降低了中央细胞中F-actin的动态向内运动，表明formmin在中心细胞中F-actin向内运动所需的肌动蛋白成核中起主要作用。
In the female gamete of flowering plants, sperm nuclear migration is controlled by a constant inward movement of actin filaments (F-actin) for successful fertilization. This dynamic F-actin movement is ARP2/3-independent, raising the question of how actin nucleation and polymerization is controlled in the female gamete. Using confocal microscopy live-cell imaging in combination with a pharmacological approach, we assessed the involvement of another group of actin nucleators, formins, in F-actin inward movement in the central cell of Arabidopsis thaliana. We identify that the inhibition of the formin function, by formin inhibitor SMIFH2, significantly reduced the dynamic inward movement of F-actin in the central cell, indicating that formins play a major role in actin nucleation required for F-actin inward movement in the central cell.