Back to the future—The value of single protein species investigations
Back to the future—The value of single protein species investigations
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回到未来——单一蛋白质物种研究的价值
作者：
P. Jungblut
在蛋白质组学中，在过去的几年里，有一个重点是高通量和达到大量的鉴定蛋白质与蛋白质表达的基本话语。为了避免产生纯列表的印象，通常尝试将两种生物情况之间的蛋白质数量变化与不同的途径或蛋白质相互作用相关联。这篇论文是基于两个简化，这大大限制了蛋白质组学的适用性：（i）它足以从几个酶消化产物中定量蛋白质;（ii）生物学的情况是充分描述的，如果一个肽与其PTM被确定，导致修改的肽与数据量的长列表，这不再是出版物的读者访问。Kimura等人（Proteomics 2013，13，3167-3174）对酵母中蛋白酶体亚基Rpt 1的N-末端甲基化的阐明代表了对一种蛋白质的关注，显示了固体化学分析的价值和完整的数据文件，并为基于蛋白质物种形成论述的蛋白质组学铺平了道路。
In proteomics, in the past years, there was a focus on high throughput and reaching of large numbers of identified proteins with the basic discourse of protein expression. To avoid the impression of producing pure lists attempts are usually made to correlate proteins changed in amount between two biological situations to different pathways or protein interactions. This discourse is based on two simplifications, which limit the applicability of proteomics drastically: (i) it is sufficient to quantify a protein from several enzymatic digestion products; (ii) a biological situation is sufficiently described, if a peptide with its PTM is identified, resulting in long lists of modified peptides with data amounts, which are not anymore made accessible for the reader of a publication. The elucidation of N‐terminal methylation of proteasome subunit Rpt1 in yeast by Kimura et al. (Proteomics 2013, 13, 3167–3174), which represents the focus on one protein, shows the value of solid chemical analysis with a complete data documentation and paves the way to proteomics based on the protein speciation discourse.