QUANTITATION OF BACTERIA IN CEREBROSPINAL-FLUID AND BLOOD OF CHILDREN WITH MENINGITIS AND ITS DIAGNOSTIC-SIGNIFICANCE
QUANTITATION OF BACTERIA IN CEREBROSPINAL-FLUID AND BLOOD OF CHILDREN WITH MENINGITIS AND ITS DIAGNOSTIC-SIGNIFICANCE
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DOI：
10.1128/jcm.19.2.187-190.1984
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发表时间：
1984-01-01
影响因子：
9.4
通讯作者：
DRYJA, D
中科院分区：
文献类型：
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作者：
LASCOLEA, LJ;DRYJA, D
本文对脑膜炎患儿的脑脊液标本进行了微生物浓度检测，并与菌血症水平、镜检结果、中性粒细胞浓度之间的关系进行了分析。共分析了2031份连续的脑脊液样本，其中63份(3.1%)来自相同数量的患者的培养阳性。我们观察到，在b型流感嗜血杆菌、无乳链球菌、肺炎链球菌和脑膜炎奈瑟菌阳性的脑脊液标本中，85%的标本计数超过10(3)cfu/ml，其中56%的标本超过10(5)cfu/ml。脑脊液和血液中的细菌数量之间存在相关性。例如，在总共22例脑脊液中流感嗜血杆菌计数超过10(3)cfu/ml的患者中，16例(73%)的菌血症水平超过10(3)cfu/ml。此外，还注意到细菌浓度对显微镜的灵敏度有很大影响。在10(3)~10(5)cfu/ml的范围内，阳性结果的百分率从25%上升到60%，当浓度大于10(5)cfu/ml时，阳性结果的百分率上升到97%，而且脑脊液中细菌浓度与镜检中多形核白细胞的数量之间存在显著的相关性(P<0.01)。
Cerebrospinal fluid (CSF) specimens from pediatric patients with meningitis were examined for their concentration of microbes and the relationship of this count to the bacteremia levels, microscopy results, and polymorphonuclear leukocyte concentration. A total of 2,031 consecutive CSF specimens were analyzed, of which 63 (3.1%) were positive by culture from the same number of patients. We observed that 85% of the total CSF specimens positive for Haemophilus influenzae type b, Streptococcus agalactiae, Streptococcus pneumoniae, and Neisseria meningitidis had counts in excess of 10(3) CFU/ml, with 56% of the specimens exceeding 10(5) CFU/ml. A correlation existed between the number of organisms present in the CSF and blood. For example, from a total of 22 patients who had counts of H. influenzae greater than 10(3) CFU/ml in the CSF, 16 or 73% had levels of bacteremia greater than 10(3) CFU/ml. It was also noted that the bacterial concentration had a profound effect on the sensitivity of microscopy. The percentage of positive results increased from 25% with less than or equal to 10(3) CFU/ml to 60% in the range of greater than 10(3) to 10(5) CFU/ml and to 97% at concentrations of greater than 10(5) CFU/ml. Furthermore, a significant correlation (P less than 0.01) was noted between the concentration of bacteria in the CSF and the number of polymorphonuclear leukocytes observed on microscopy.