Complete genome sequence and phylogenetic relationships of tobacco streak virus causing groundnut stem necrosis disease in India.
Complete genome sequence and phylogenetic relationships of tobacco streak virus causing groundnut stem necrosis disease in India.
复制标题
导致印度花生茎坏死病的烟草条纹病毒的完整基因组序列和系统发育关系。
作者：
Daliyamola;Jailani;A.A.K. Vemana;K.;Roy;A.;Krishnareddy;M.;Kobayashi;K. Mandal;B.
烟草条纹病毒（TSV, ilarvirusfamilybromoviridae）已知自2000年以来在印度南部引起花生（Arachis hypogaea）茎坏死病（SND）。到目前为止，感染花生的TSV分离物尚未根据完整的基因组序列进行鉴定。在这项研究中，TSV从印度南部SND的热点地区Kadiri的一株自然侵染的花生植物中分离出来。2014年秋收季节，某试验田花生发生叶、茎、芽萎黄坏死。在这种情况下，从现场收集的48个有这些症状的样本中，ELISA样本中TSV检测呈阳性的百分比。其中一个分离物GN-Kad是从豇豆cv的单一病株中分离得到的。通过连续接液接种C-152。对该分离物外壳蛋白基因（717个核苷酸）的克隆和测序结果表明，该分离物与印度不同作物分离物具有较高的同源性（98 ~ 99%）。对豇豆（品种C-152、Pusa Komal、Pusa Sukomal和Krishi Kanchan）、豆荚和向日葵接种液后，分离物产生局部坏死环或静脉坏死；而在本烟中产生全身褪绿斑驳症状。病毒基因组的三个片段（RNA 1、RNA 2和RNA 3）分别含有3523、2903和2232个核苷酸。该分离株的总基因组序列（8639 nt）与来自澳大利亚、印度和美国的其他7株分离株的核苷酸序列同源性为77-99%。GN-Kad与印度报道的秋葵和南瓜分离株具有非常密切的系统发育关系。本报告首次对花生茎坏死病相关TSV的分子特征进行了全面研究。
Tobacco streak virus (TSV, genusIlarvirusfamilyBromoviridae) is known to cause stem necrosis disease (SND) in groundnut (Arachis hypogaea) since 2000 in Southern India. The TSV isolate infecting groundnut so far has not been characterized based on the complete genome sequence. In this study, TSV was isolated from a naturally infecting groundnut plant in Kadiri, the hot-spot of the SND in southern India. During theKharifseason of 2014, groundnut plants in an experimental field were affected with chlorosis and necrosis in leaf, stem and buds. The cent percent of the 48 samples with these symptoms collected from the field tested positive for TSV in ELISA samples in this context. One isolate, GN-Kad was established from a single lesion on cowpea cv. C-152 through successive sap inoculation. Cloning and sequencing of coat protein gene (717 nucleotides) of the isolate showed high sequence identity (98–99%) with the TSV isolates reported from different crops in India. The isolate produced local necrotic rings or veinal necrosis following sap inoculation to cowpea (cultivars C-152, Pusa Komal, Pusa Sukomal and Krishi Kanchan), French bean and sunflower; whereas, it produced systemic chlorotic mottling symptoms inNicotiana benthamiana. The three segments of the virus genome (RNA 1, RNA 2 and RNA 3) contained 3523, 2903 and 2232 nucleotides, respectively. The overall genome sequence (8639 nt) of the present isolate shared 77–99% of nucleotide sequence identity with that of the other seven isolates reported from Australia, India and USA. The GN-Kad shared very close phylogenetic relationship with the okra and pumpkin isolates reported from India. The present report is the first comprehensive study of the molecular characterization of TSV associated with the stem necrosis disease of groundnut.