IMMUNOCYTOCHEMICAL LOCALIZATION OF A GROWTH-ASSOCIATED PROTEIN (GAP-43) IN RAT ADRENAL-GLAND
IMMUNOCYTOCHEMICAL LOCALIZATION OF A GROWTH-ASSOCIATED PROTEIN (GAP-43) IN RAT ADRENAL-GLAND
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DOI：
10.1007/bf00318824
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发表时间：
1994-03-01
影响因子：
3.6
通讯作者：
PRIESTLEY, JV
中科院分区：
文献类型：
--
作者：
COSTA, JJL;AVERILL, S;PRIESTLEY, JV
应用单、双标记免疫细胞化学技术，对生长相关蛋白GAP-43在肾上腺组织中进行了光镜和电镜定位。髓质内可见GAP-43免疫荧光染色阳性的嗜铬细胞和大量免疫荧光纤维。GAP-43免疫反应阳性纤维也形成了一个丛下的胶囊，穿过皮质和分枝的网状。双标记切片显示GAP-43与酪氨酸羟化酶和多巴胺-β-羟化酶免疫反应性嗜铬细胞亚群共表达。GAP-43和降钙素基因相关肽（CGRP）的双色免疫荧光显示，一些差距-43免疫反应纤维也表达CGRP。包埋前电子显微镜显示GAP-43免疫反应与质膜和细胞质的去甲肾上腺素产生的嗜铬细胞，并与非髓鞘形成的雪旺细胞的过程。还观察到免疫反应性无髓鞘轴突和终末。免疫染色的终末与嗜铬细胞形成对称的突触接触。免疫反应性无髓纤维和小终端存在于皮质。我们的研究结果表明，GAP-43表达在去甲肾上腺素能嗜铬细胞和各种类型的神经纤维支配肾上腺。这些纤维的可能来源包括支配嗜铬细胞的节前交感神经纤维、皮质中的节后交感神经纤维和含有CGRP的感觉纤维。
We have localized at light and electron-microscopic level the growth-associated protein GAP-43 in adrenal gland using single and double labelling immunocytochemistry. Clusters of GAP-43-immunofluorescent chromaffin cells and many immunofluorescent fibres were observed in the medulla. GAP-43-immunoreactive fibres also formed a plexus under the capsule, crossed the cortex and ramified in the zona reticulata. Double labelled sections showed the coexpression of GAP-43 with a subpopulation of tyrosine hydroxylase- and of dopamine-beta-hydroxylase-immunoreactive chromaffin cells. Dual colour immunofluorescence for GAP-43 and calcitonin gene-related peptide (CGRP) revealed that some of the GAP-43-immunoreactive fibres also express CGRP. Pre-embedding electron microscopy showed GAP-43 immunoreactivity associated with the plasma membranes and cytoplasm of noradrenaline-producing chromaffin cells, and with processes of nonmyelin-forming Schwann cells. Immunoreactive unmyelinated axons and terminals were also observed. The immunostained terminals made symmetrical synaptic contacts with chromaffin cells. Immunoreactive unmyelinated fibres and small terminals were present in the cortex. Our results show that GAP-43 is expressed in noradrenergic chromaffin cells and in various types of nerve fibres that innervate the adrenal. Likely origins for these fibres include preganglionic sympathetic fibres which innervate chromaffin cells, postganglionic sympathetic fibres in the cortex, and CGRP containing sensory fibres.