Cerebroprotective effect of resveratrol through antioxidant and anti-inflammatory effects in diabetic rats
Cerebroprotective effect of resveratrol through antioxidant and anti-inflammatory effects in diabetic rats
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DOI：
10.1007/s00210-013-0871-2
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发表时间：
2013-08-01
影响因子：
3.6
通讯作者：
Prabhakar, Orsu
中科院分区：
文献类型：
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作者：
Prabhakar, Orsu
氧化应激和炎症反应与脑缺血/再灌注损伤和糖尿病并发症有关。本研究旨在评价白藜芦醇是否通过抗氧化和抗炎作用对糖尿病大鼠的肾脏保护作用。采用双侧颈总动脉阻断（30 min）再灌注（4 h）的方法，建立糖尿病大鼠脑梗死模型。用链脲佐菌素（50 mg/kg）一次性腹腔注射诱发糖尿病。将糖尿病动物分为正常组、假手术组、缺血再灌注组和白藜芦醇治疗组（5、10、20和30 mg/kg）。这些用于估计脑梗死。此外，选择20 mg/kg剂量估计氧化应激标志物（丙二醛、超氧化物歧化酶和过氧化氢酶）。评估炎症标志物如TNF-α、IL-6、IL-10和髓过氧化物酶，并研究组织学特征。白藜芦醇产生剂量依赖性降低脑梗死百分比。使用20 mg/kg剂量的白藜芦醇，氧化应激标志物和炎症标志物如丙二醛、TNF-α、IL-6和髓过氧化物酶的水平降低，并且抗氧化剂和抗炎标志物如过氧化氢酶、超氧化物歧化酶和IL-10的水平显著增加。在本研究中，我们发现白藜芦醇在糖尿病大鼠脑中的神经保护作用的机制涉及抗氧化和抗炎作用。
Oxidative stress and inflammation have been implicated in cerebral ischemia/reperfusion injury and complication of diabetes. The present study was designed to evaluate whether resveratrol has cerebroprotective action through antioxidant and anti-inflammatory actions in diabetic rats. Bilateral common carotid artery occlusion (30 min) and reperfusion (4 h) was employed to induce cerebral infarction in diabetic Wistar rats. Diabetes was induced by streptozocine (50 mg/kg) intraperitoneally at once. Diabetic animals were divided into groups as: normal, sham, ischemia-reperfusion, and resveratrol-treated (5, 10, 20, and 30 mg/kg). These were used for estimation of cerebral infarction. Furthermore, 20 mg/kg dose was selected for estimation of oxidative stress markers (malondialdehyde, superoxide dismutase, and catalase). Inflammatory markers like TNF-alpha, IL-6, IL-10, and myeloperoxidase were estimated and histological characters were studied. Resveratrol produced dose-dependent reduction in percent cerebral infarction. With resveratrol of 20 mg/kg dose, levels of oxidative stress markers and inflammatory markers like malondialdehyde, TNF-alpha, IL-6, and myeloperoxidase were reduced and there was a significant increase in the levels of antioxidant and anti-inflammatory markers like catalase, superoxide dismutase, and IL-10. In the present study, we found that mechanism(s) responsible for the cerebroprotective effect of resveratrol in the diabetic rat brain involves antioxidant and anti-inflammatory actions.