Accumulation of apolipoprotein C-I-rich and cholesterol-rich VLDL remnants during exaggerated postprandial triglyceridemia in normolipidemic patients with coronary artery disease
Accumulation of apolipoprotein C-I-rich and cholesterol-rich VLDL remnants during exaggerated postprandial triglyceridemia in normolipidemic patients with coronary artery disease
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DOI：
10.1161/01.cir.101.3.227
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发表时间：
2000-01-25
期刊：
影响因子：
37.8
通讯作者：
Hamsten, A
中科院分区：
文献类型：
--
作者：
Björkegren, J;Boquist, S;Hamsten, A
背景：餐后高血脂在血脂正常的冠心病患者中很常见。富含甘油三酯的脂蛋白（TRL）组成的改变可能是这种代谢紊乱的基础。然而，组成的极低密度脂蛋白（VLDL），这是最丰富的餐后TRLs，从来没有被定义在CAD patients.Methods和Results-We研究餐后变化的数量和组成的VLDL在中年，血脂正常的CAD患者和对照组。通过密度梯度超离心将14例患者和14例对照受试者（年龄45 - 55岁）的TRL细分为Svedberg浮选率（Sf）>400、60 - 400和20 - 60。通过免疫亲和层析从乳糜微粒残留物中分离出VLDL。在CAD患者中，餐后甘油三酯和大剂量甘油三酯浓度（Sf 60至400）VLDL颗粒升高，此外，他们的餐后大VLDL富含载脂蛋白（apo）C-I，（Sf 20 - 60）VLDL残余物富含载脂蛋白C-I和胆固醇。结论：血脂正常的CAD患者餐后甘油三酯的紊乱处理涉及载脂蛋白C-I的积累。富含大的VLDL颗粒和产生小的、富含载脂蛋白C-I和胆固醇的VLDL残余物。
Background-Exaggerated postprandial triglyceridemia is common in normolipidemic patients with coronary artery disease (CAD). Alterations in the composition of triglyceride-rich lipoproteins (TRLs) are likely to underlie this metabolic disturbance. However, the composition of very-low-density lipoproteins (VLDLs), which are the most abundant postprandial TRLs, has never been defined in CAD patients.Methods and Results-We examined postprandial changes in the number and composition of VLDLs in middle-aged, normolipidemic CAD patients and control subjects. TRLs from 14 patients and 14 control subjects aged 45 to 55 years were subfractionated by density gradient ultracentrifugation into Svedberg flotation rate (Sf) fractions >400, 60 to 400, and 20 to 60. The VLDLs were separated from chylomicron remnants by immunoaffinity chromatography, In CAD patients, the postprandial concentrations of triglycerides and large (Sf 60 to 400) VLDL particles were elevated, In addition, their postprandial large VLDLs were enriched in apolipoprotein (apo) C-I and their postprandial small (Sf 20 to 60) VLDL remnants were enriched with apo C-I and cholesterol.Conclusions-Perturbed handling of postprandial triglycerides in normolipidemic CAD patients involves the accumulation of apo C-I-rich large VLDL particles and the generation of small, apo C-I- and cholesterol-rich VLDL remnants.