ISOLATION OF CYTOTOXIC SUBSTANCE, KALAFUNGIN FROM AN ALKALOPHILIC ACTINOMYCETE, NOCARDIOPSIS-DASSONVILLEI SUBSP PRASINA
ISOLATION OF CYTOTOXIC SUBSTANCE, KALAFUNGIN FROM AN ALKALOPHILIC ACTINOMYCETE, NOCARDIOPSIS-DASSONVILLEI SUBSP PRASINA
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DOI：
10.1248/cpb.38.2299
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发表时间：
1990-08-01
影响因子：
1.7
通讯作者：
INAMORI, Y
中科院分区：
文献类型：
--
作者：
TSUJIBO, H;SAKAMOTO, T;INAMORI, Y
OPC-553是一株嗜碱放线菌，被认为是Nocardiopsis dassonvillei subsp. Prasina产生的细胞毒物质TS-1在体外对小鼠白血病细胞L5178 Y有明显的抑制作用。TS-1对该细胞的细胞毒性非常强，其ID 50为0.018 μ g/ml。通过与真实样品的光谱数据的直接比较，TS-1被鉴定为已经从Streptomyces tanashiensis的培养液中分离的抗真菌抗生素卡拉芬净。然而，卡拉芬净从嗜碱放线菌中的分离及其细胞毒作用尚属首次报道。
An alkalophilic actinomycete, strain OPC-553 regarded as Nocardiopsis dassonvillei subsp. prasina, produced the cytotoxic substance, TS-1, which showed a marked inhibitory activity L5178Y mouse leukemic cell in vitro. The cytotoxicity of TS-1 on this cell was very strong and its ID50 was 0.018 .mu.g/ml. Through direct comparison of its spectral data with those of an authentic sample, TS-1 was identified as the antifungal antibiotic, kalafungin, already isolated from the culture broth of Streptomyces tanashiensis. However, the isolation of kalafungin from an alkalophilic actinomycete and its cytotoxicity are reported for the first time in this paper.