SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal
SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal
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DOI：
10.4161/epi.23416
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发表时间：
2013-02-01
期刊：
影响因子：
3.7
通讯作者：
Richard, Stephane
中科院分区：
文献类型：
--
作者：
Binda, Olivier;Sevilla, Ana;Richard, Stephane
组蛋白H2A变体H2AZ是一种必需的染色质信号传导因子。在此，我们报告，H2AZ是单甲基化的赖氨酸7（H2AZK7（me1））的赖氨酸甲基转移酶SETD6。我们观察到H2AZ的甲基化在小鼠胚胎干细胞（mESCs）的细胞分化中显著增加。H2AZK7（me1）和抑制性H3K27（me3）标记被发现附近的分化标记基因的转录起始位点，但被删除后，视黄酸诱导的细胞分化。mESC中Setd6的缺失导致细胞分化、自我更新受损和克隆形成性差。这些发现表明mESC需要Setd6进行自我更新，并将H2AZK7（me1）描绘为细胞分化的标志物。
The histone H2A variant H2AZ is an essential chromatin signaling factor. Herein, we report that H2AZ is monomethylated at lysine 7 (H2AZK7(me1)) by the lysine methyltransferase SETD6. We observed that methylation of H2AZ increased noticeably upon cellular differentiation of mouse embryonic stem cells (mESCs). H2AZK7(me1) and the repressive H3K27(me3) mark were found near the transcriptional start sites of differentiation marker genes, but were removed upon retinoic acid-induced cellular differentiation. The depletion of Setd6 in mESCs led to cellular differentiation, compromised self-renewal, and poor clonogenicity. These findings demonstrate that mESCs require Setd6 for self-renewal and portray H2AZK7(me1) as a marker of cellular differentiation.