Putative diazepam binding inhibitor peptide: cDNA clones from rat.
Putative diazepam binding inhibitor peptide: cDNA clones from rat.
复制标题
推定的地西泮结合抑制肽：来自大鼠的 cDNA 克隆。
DOI：
10.1073/pnas.83.19.7221
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发表时间：
1986
影响因子：
11.1
通讯作者：
Brosius,J
中科院分区：
文献类型：
--
作者：
Mocchetti,I;Einstein,R;Brosius,J
已从来自大鼠下丘脑、全脑和肝脏的噬菌体λ重组文库中分离出对应于已被证明是内源地西泮结合抑制剂并可充当苯二氮卓/β-咔啉受体的生理配体的多肽的cDNA克隆。 The clones contain an open reading frame corresponding to 87 amino acids.不存在信号序列。除了各个大脑区域中的高水平 mRNA 外，RNA 印迹分析还揭示了许多外周器官（例如睾丸、肾脏、肝脏和心脏）中存在丰富的地西泮结合抑制剂 mRNA，这些器官已知富含外周苯二氮卓类识别位点。所有检查组织中 mRNA 的大小约为 0.7 kb。基因组 DNA 的 Southern 印迹分析表明大鼠体内存在约 6 个基因，其中一些可能是假基因。
cDNA clones corresponding to the polypeptide that has been shown to be an endogenous diazepam binding inhibitor and may act as a physiological ligand for the benzodiazepine/beta-carboline receptor have been isolated from bacteriophage lambda recombinant libraries from rat hypothalamus, total brain, and liver. The clones contain an open reading frame corresponding to 87 amino acids. A signal sequence is not present. In addition to high levels of mRNA in various brain regions, RNA blot analysis reveals an abundance of diazepam binding inhibitor mRNA in many peripheral organs (e.g., testes, kidney, liver, and heart) that are known to be rich in peripheral benzodiazepine recognition sites. The size of the mRNA in all tissue examined is approximately 0.7 kilobase. Southern blot analysis of genomic DNA suggests the presence of about six genes in the rat, some of which may be pseudogenes.