Variation in the polyadenylylation site of bovine prolactin mRNA.
Variation in the polyadenylylation site of bovine prolactin mRNA.
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牛催乳素 mRNA 聚腺苷酸化位点的变异。
DOI：
10.1073/pnas.79.2.223
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发表时间：
1982
影响因子：
11.1
通讯作者：
Rottman,FM
中科院分区：
文献类型：
--
作者：
Sasavage,NL;Smith,M;Gillam,S;Woychik,RP;Rottman,FM
用序列为d（pT 8-N-N '）的寡脱氧核苷酸对牛催乳素（bPRL）mRNA的poly（A）位点进行了分阶段诱导。在bPRL mRNA中存在多个poly（A）-邻近序列，这通过产生至少三个d（pT 8-N-N '）序列的特异性链终止片段来指示。通过在bPRL mRNA模板上起始cDNA合成产生的序列条带与d（pT 8-A-G）、d（pT 8-G-A）和d（pT 8-C-G）的比较，揭示了相同片段的移位模式。凝胶上相关序列条带的位置变化表明，三种主要bPRL mRNA种类的长度差异发生在12个核苷酸的跨度内。用3条d（pT 8-N-N ′）引物进行的序列分析表明bPRL mRNA 3 ′非编码区的核苷酸序列完全相同，表明mRNA分子长度不均一。多聚腺苷酸位点的存在通过测定含有寡脱氧核苷酸引物预测的两个主要聚腺苷酸相邻序列的bPRL cDNA克隆的核苷酸序列来证实。含有这些多聚（A）-添加位点的mRNA分子被证明存在于从单个垂体获得的bPRL mRNA中。bPRL mRNA的poly（A）连接的变化可能反映了mRNA 3'末端的加工事件。
The poly(A) site of bovine prolactin (bPRL) mRNA was examined by phased priming of cDNA synthesis with oligodeoxynucleotides of the general sequence d(pT8-N-N'). The existence of multiple poly(A)-adjacent sequences in bPRL mRNA was indicated by the production of specific chain-termination fragments with at least three d(pT8-N-N') sequences. Comparison of the sequence bands produced by initiation of cDNA synthesis on the bPRL mRNA template with d(pT8-A-G), d(pT8-G-A), and d(pT8-C-G) revealed a shifted pattern of identical fragments. The shift in position of related sequence bands on the gel suggested that the difference in length of the three major bPRL mRNA species occurred within a span of 12 nucleotides. Sequence analysis conducted with the three d(pT8-N-N') primers gave identical nucleotide sequences for the 3' noncoding region of the bPRL mRNA species and suggested that the mRNA molecules were heterogeneous in length. The existence of multiple poly(A) sites was confirmed by determination of the nucleotide sequence of bPRL cDNA clones containing two of the major poly(A)-adjacent sequences predicted by the oligodeoxynucleotide primers. The mRNA molecules containing these multiple poly(A)-addition sites were shown to be present in the bPRL mRNA obtained from a single pituitary gland. The variation in the poly(A) junction of bPRL mRNA may be a reflection of the processing events at the 3' terminus of mRNAs.