POLY(D2NH2A-DT) - TWO-DIMENSIONAL NMR SHOWS A B TO A CONVERSION IN HIGH SALT
POLY(D2NH2A-DT) - TWO-DIMENSIONAL NMR SHOWS A B TO A CONVERSION IN HIGH SALT
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DOI：
10.1021/bi00346a064
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
MILES, HT
中科院分区：
文献类型：
--
作者：
BORAH, B;COHEN, JS;MILES, HT
聚（d2 NH 2 A-dT）在高盐中的结构与低盐中存在的B形式明显不同。二维核奥弗豪泽效应（2D NOE）NMR谱确定高盐形式的构象不是Z。在2D NOE光谱中观察到的交叉峰和常见DNA构象的估计质子间距离的相关性仅与A形式或密切相关的结构一致。这种解释也与在高盐形式的聚（d2 NH 2A-dT）和含有2NH 2A的A型核糖均聚物螺旋中观察到的负圆形二向色带一致。
Poly(d2NH2A-dT) from a structure in high salt that is clearly distinct from the B form present in low salt. Two-dimensional nuclear Overhauser effect (2D NOE) NMR spectra establish that the conformation of the high-salt form is not Z. Correlations of observed cross peaks in the 2D NOE spectra and estimated interproton distances of the common DNA conformations are consistent only with an A form or closely related structure. This interpretation is also consistent with the negative circular dichroic band observed in the high-salt form of poly(d2NH2A-dT) and in A-form ribohomopolymer helices containing 2NH2A.