Water molecular structure underpins extreme desiccation tolerance of the resurrection plant Haberlea rhodopensis
Water molecular structure underpins extreme desiccation tolerance of the resurrection plant Haberlea rhodopensis
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DOI：
10.1038/s41598-019-39443-4
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发表时间：
2019-02-28
影响因子：
4.6
通讯作者：
Djilianov, Dimitar
中科院分区：
文献类型：
--
作者：
Kuroki, Shinichiro;Tsenkova, Roumiana;Djilianov, Dimitar
玫瑰花是一种具有极高脱水耐性的复苏植物。即使经过长时间的几乎完全干燥，其生理功能恢复后不久，重新浇水。为了确定有助于其显着的干旱胁迫耐受性的生理策略，我们用近红外光谱法，以调查这种植物的叶片中的水的状态，并将其与其相对的，非复活的植物物种Deinostigma eberhardtii进行比较。在这里，我们表明，使用一种新的aquaphotomics光谱分析，H。rhodopensis在脱水过程中进行水分子结构的动态调节，在完全干燥阶段，自由水分子急剧减少，具有4个氢键的水分子增加，并且大量积累水二聚体。我们的研究结果表明，水结构的变化反映了主要代谢产物和抗氧化剂的变化，它们共同构成了一个强大的防御系统的基础上的脱水耐受性的复苏植物，而水二聚体可能持有特殊的重要性，“干燥而不死”的能力。
Haberlea rhodopensis is a resurrection plant with an extremely high desiccation tolerance. Even after long periods of almost full desiccation, its physiological functions are recovered shortly upon re-watering. In order to identify physiological strategies which contribute to its remarkable drought stress tolerance we used near infrared spectroscopy to investigate the state of water in the leaves of this plant and compared it to its relative, non-resurrection plant species Deinostigma eberhardtii. Here we show, using a novel aquaphotomics spectral analysis, that H. rhodopensis performs a dynamic regulation of water molecular structure during dehydration directed at drastic decrease of free water molecules, increase of water molecules with 4 hydrogen bonds, and a massive accumulation of water dimers in the full desiccation stage. Our findings suggest that changes in water structure mirror the changes in major metabolites and antioxidants which together constitute a robust defense system underlying the desiccation tolerance of the resurrection plant, while the water dimer may hold special importance for the "drying without dying" ability.