Identification and utilization of a new Erysiphe necator isolate NAFU1 to quickly evaluate powdery mildew resistance in wild Chinese grapevine species using detached leaves
Identification and utilization of a new Erysiphe necator isolate NAFU1 to quickly evaluate powdery mildew resistance in wild Chinese grapevine species using detached leaves
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DOI：
10.1016/j.plaphy.2015.11.003
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发表时间：
2016-01-01
影响因子：
6.5
通讯作者：
Wen, Ying-Qiang
中科院分区：
文献类型：
--
作者：
Gao, Yu-Rong;Han, Yong-Tao;Wen, Ying-Qiang
全世界栽培葡萄藤的最经济重要的疾病是由活体营养真菌病原体葡萄白粉菌（Erysiphe necator）引起的白粉病。为了将有效的遗传抗性整合到栽培葡萄藤中，已经进行了包括野生种在内的多种葡萄属种质的许多抗病筛选以鉴定白粉病抗性，但是结果一直不一致。在这里，一个新的白粉病分离物，是感染葡萄藤，命名为葡萄白粉菌NAFU 1（En。NAFU 1），并通过从病原体核糖体DNA序列的内转录间隔区（ITS）推断的同源性来鉴定和表征。比较了三种经典方法对En的维持。NAFU 1，最方便的方法是离体叶片上的维护和感染叶片接触繁殖。此外，控制接种En。利用台盼蓝染色法对57份中国野生葡萄藤材料的离体叶片进行NAFU 1鉴定，以快速评价葡萄的白粉病抗性。将结果与该领域以前的自然流行病进行了比较。在筛选的材料中，接种了En. NAFU 1的抗性占22.8%，中抗占33.3%，感病占43.9%。本文评估的种质中没有一个对感染免疫。这些结果支持了先前的研究结果，证明了存在种族特异性抵抗E。中国野生葡萄藤中的钩虫。野生中国葡萄藤对内生真菌的抗性。NAFU 1可能是由于程序性细胞死亡。目前的结果表明，En. NAFU 1菌株可用于不同葡萄属种质资源的大规模白粉病抗性筛选以及葡萄与病原菌互作的研究。(C)2015年Elsevier Masson SAS。All rights reserved.
The most economically important disease of cultivated grapevines worldwide is powdery mildew caused by the biotrophic fungal pathogen Erysiphe necator. To integrate effective genetic resistance into cultivated grapevines, numerous disease resistance screens of diverse Vitis germplasm, including wild species, have been conducted to identify powdery mildew resistance, but the results have been inconsistent. Here, a new powdery mildew isolate that is infectious on grapevines, designated Erysiphe necator NAFU1 (En. NAFU1), was identified and characterized by phylogeny inferred from the internal transcribed spacer (ITS) of pathogen ribosomal DNA sequences. Three classical methods were compared for the maintenance of En. NAFU1, and the most convenient method was maintenance on detached leaves and propagation by contact with infected leaves. Furthermore, controlled inoculations of En. NAFU1 were performed using detached leaves from 57 wild Chinese grapevine accessions to quickly evaluate powdery mildew resistance based on trypan blue staining of leaf sections. The results were compared with previous natural epidemics in the field. Among the screened accessions inoculated with En. NAFU1, 22.8% were resistant, 33.3% were moderately resistant, and 43.9% were susceptible. None of the accessions assessed herein were immune from infection. These results support previous findings documenting the presence of race-specific resistance to E. necator in wild Chinese grapevine. The resistance of wild Chinese grapevine to En. NAFU1 could be due to programmed cell death. The present results suggest that En. NAFU1 isolate could be used for future large-scale screens of resistance to powdery mildew in diverse Vitis germplasms and investigations of the interaction between grapevines and pathogens. (C) 2015 Elsevier Masson SAS. All rights reserved.