BTH treatment caused physiological, biochemical and proteomic changes of muskmelon (Cucumis melo L.) fruit during ripening
BTH treatment caused physiological, biochemical and proteomic changes of muskmelon (Cucumis melo L.) fruit during ripening
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DOI：
10.1016/j.jprot.2015.03.006
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发表时间：
2015-04-29
影响因子：
3.3
通讯作者：
Shang, Qi
中科院分区：
文献类型：
--
作者：
Li, Xin;Bi, Yang;Shang, Qi
苯并-（1，2，3）-噻二唑-7-硫代甲酸S-甲酯（BTH）是一种能诱导多种作物抗病性的化学植物诱导剂。本研究以跃变型果实甜瓜为材料，对其生长发育进行了研究。用0.1g/L的BTH处理郁金香（郁金香），研究其成熟过程中生理生化和蛋白质含量的变化。结果表明，BTH处理提高了呼吸强度，降低了可滴定酸含量，延缓了果实硬度和抗坏血酸含量的下降。BTH处理后，果实成熟初期乙烯释放量增加，6d后下降。在双向电泳分离的蛋白质点中，有69个蛋白质点在BTH处理后发生了显著的丰度变化。使用MALDI-TOF/TOF通过对NCBInr数据库进行爆破来鉴定69个点中的52个。功能分类表明，所鉴定的蛋白质种类与防御和胁迫反应、蛋白质合成、目的地和储存、能量代谢、初级代谢、细胞结构、次级代谢、信号转导和转运蛋白有关。本研究综述了BTH处理后甜瓜果实成熟过程中的主要生理生化和蛋白质组学变化，为保持果实品质和延缓果实成熟衰老提供了潜在的有用信息。生物学意义本研究为从蛋白质组学水平阐明BTH处理对甜瓜果实成熟的调控机制提供了新的蛋白质组学证据，为进一步研究BTH处理果实品质与诱导抗病性的关系提供了有价值的参考。(C)2015 Elsevier B. V.版权所有。
Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) is a chemical plant elicitor capable of inducing disease resistance in many crops. In this study, the climacteric fruit muskmelon (cv. Yujinxiang) was treated with BTH at 0.1 g/L for assaying the changes in physiology, biochemistry and protein profile during ripening. The results showed that BTH treatment enhanced respiration rate, while reduced titratable acid content and retarded the decline of fruit firmness and ascorbic acid content. Ethylene production increased after BTH treatment at early stages of ripening, but decreased after 6 days of treatment. Of the detected protein spots separated by means of 2-DE, 69 spots changed in abundance significantly after BTH treatment. Fifty-two spots out of 69 were identified using MALDI-TOF/TOF by blasting against NCBInr database. Functional classification revealed that the protein species identified were related to defense and stress responses, protein synthesis, destination and storage, energy metabolism, primary metabolism, cell structure, secondary metabolism, signal transduction and transporters. This study demonstrates an overview of major physiological, biochemical and proteomic changes in muskmelon fruit during ripening after BTH treatment and provides potentially useful information for maintaining fruit quality and delaying the ripening and senescence process.Biological significanceThe study offers new proteomic evidences for elucidating the regulatory mechanism of muskmelon fruit ripening by BTH treatment at proteomic level, and provides a valuable reference for further research on the relationship between fruit quality and induction disease resistance in BTH-treated fruits. (C) 2015 Elsevier B.V. All rights reserved.