DNA-gold nanorod conjugates for remote control of localized gene expression by near infrared irradiation
DNA-gold nanorod conjugates for remote control of localized gene expression by near infrared irradiation
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DOI：
10.1021/ja0570180
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发表时间：
2006-03-22
影响因子：
15
通讯作者：
Wu, YC
中科院分区：
文献类型：
--
作者：
Chen, CC;Lin, YP;Wu, YC
利用金纳米棒连接增强型绿色荧光蛋白（EGFP）基因，远程控制该基因在活细胞中的表达。采用紫外可见光谱、电泳和透射电子显微镜（TEM）研究了飞秒近红外（NIR）激光照射前后EGFP DNA和金纳米棒（EGFP- gnr）偶联物的光学和结构特性。在近红外照射下，EGFP- gnr偶联物的金纳米棒发生形状转化，导致EGFP DNA释放。当EGFP-GNR偶联物传递到培养的HeLa细胞时，在局部暴露于近红外辐射的细胞中特异性观察到诱导的GFP表达。我们的研究结果表明，利用金纳米棒和近红外辐射作为远程控制特定细胞基因表达的手段是可行的。这种方法在生物学和医学研究中具有潜在的应用前景。
Gold nanorods were attached to the gene of enhanced green fluorescence protein (EGFP) for the remote control of gene expression in living cells. The UV-vis spectroscopy, electrophoresis, and transmission electron microscopy (TEM) were used to study the optical and structural properties of the EGFP DNA and gold nanorod (EGFP-GNR) conjugates before and after femto-second near-infrared (NIR) laser irradiation. Upon NIR irradiation, the gold nanorods of EGFP-GNR conjugates underwent shape transformation that resulted in the release of EGFP DNA. When EGFP-GNR conjugates were delivered to cultured HeLa cells, induced GFP expression was specifically observed in cells that were locally exposed to NIR irradiation. Our results demonstrate the feasibility of using gold nanorods and NIR irradiation as means of remote control of gene expression in specific cells. This approach has potential applications in biological and medical studies.