Response of normal female human osteoblasts (NHOst) to 17β-estradiol is modulated by implant surface morphology
Response of normal female human osteoblasts (NHOst) to 17β-estradiol is modulated by implant surface morphology
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DOI：
10.1002/jbm.10290
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发表时间：
2002-11-01
期刊：
影响因子：
--
通讯作者：
Schwartz, Z
中科院分区：
文献类型：
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作者：
Lohmann, CH;Tandy, EM;Schwartz, Z
具有粗糙微形貌的钛（Ti）表面可以增强体外成骨分化、局部因子产生和对成骨剂的反应，并增加体内种植体的拔牙强度。雌激素调节女性骨形成、骨吸收和骨重塑，可能对植入物的成功很重要。在这里，我们验证了雌激素调节成骨细胞对种植体表面形态反应的假设。培养原代雌性人成骨细胞在三个钛表面融合(预处理，PT - R(a) 0.60 mum；喷砂和酸蚀，SLA - R(a) 3.97 mum；和Ti等离子喷涂，TPS - R(a) 5.21 mum)，用10（-7）或10(-8)m17 β -雌二醇（E(2)）处理24 h。细胞数量随表面粗糙度的增加而减少，但对E不敏感，粗表面离体细胞和细胞层裂解物碱性磷酸酶比活性较低。在光滑表面上，E增加了这两个参数，而在粗糙表面上，E对碱性磷酸酶的刺激作用只有在测量细胞层裂解物时才明显。骨钙素水平在粗糙表面生长的细胞的条件培养基中较高；E，对塑料表面的培养没有影响，但增加了所有钛表面的骨钙素产量。tgf - β 1和PGE(2)的产生在粗糙表面增加，E(2)以协同方式增强了这种作用；在光滑表面上，E对成骨细胞对表面形貌的反应是由E调节的，在光滑表面上，E(2)只影响碱性磷酸酶，但在粗糙表面上，E(2)增加了骨钙素、tgf - β 1和PGE的水平(2)。这些结果表明，正常成人骨母细胞对表面微形貌敏感，而E(2)可以改变这种反应。(C) 2002 Wiley期刊有限公司
Titanium (Ti) surfaces with rough microtopographies enhance osteogenic differentiation, local factor production, and response to osteogenic agents in vitro and increase pullout strength of dental implants in vivo. Estrogens regulate bone formation, resorption, and remodeling in females and may be important in implant success. Here, we tested the hypothesis that estrogen modulates osteoblast response to implant surface morphology. Primary female human osteoblasts were cultured to confluence on three Ti surfaces (pretreatment, PT - R(a) 0.60 mum; sandblasted and acid-etched, SLA - R(a) 3.97 mum; and Ti plasma-sprayed, TPS - R(a) 5.21 mum) and treated for 24 h with 10(-7) or 10(-8) M 17beta-estradiol (E(2)). Cell number decreased with increasing surface roughness, but was not sensitive to E, Alkaline phosphatase specific activity of isolated cells and cell layer lysates was lower on rough surfaces. E, increased both parameters on smooth surfaces, whereas on rough surfaces, the stimulatory effect of E, on alkaline phosphatase was evident only when measuring cell layer lysates. Osteocalcin levels were higher in the conditioned media of cells grown on rough surfaces; E, had no effect in cultures on the plastic surfaces, but increased osteocalcin production on all Ti surfaces. TGF-beta1 and PGE(2) production was increased on rough surfaces, and E(2) augmented this effect in a synergistic manner; on smooth surfaces, there was no change in production with E, The response of osteoblasts to surface topography was modulated by E, On smooth surfaces, E(2) affected only alkaline phosphatase, but on rough surfaces, E(2) increased levels of osteocalcin, TGF-beta1, and PGE(2). These results show that normal adult human fen-tale osteoblasts are sensitive to surface microtopography and that E(2) can alter this response. (C) 2002 Wiley Periodicals, Inc.