A panel of radiation hybrids for human chromosome 8.
A panel of radiation hybrids for human chromosome 8.
复制标题
一组人类 8 号染色体的辐射杂交体。
DOI：
10.1006/geno.1994.1244
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发表时间：
1994
期刊：
影响因子：
4.4
通讯作者：
Wagner,M
中科院分区：
文献类型：
--
作者：
Sapru,M;Gu,J;Gu,X;Smith,D;Yu,CE;Wells,D;Wagner,M
我们已经开发出一组含有8号染色体片段的辐射杂交体，作为唯一的人类材料。每个细胞系的人类染色体含量是根据8号染色体特异的序列标记位点(STSS)的有序图谱确定的。在每个细胞系中发现了8号染色体的1-4个片段，平均25%的STSS保留在每个细胞系中。对一个辐射杂交种的亚克隆进行了检查，以确定同一品系中的所有细胞在8号染色体序列内容方面是否都是同源的。亚无性系之间存在较大的差异，不同无性系对单个STS的保留率从5%到100%不等。此外，还发现了沿一个大的染色体片段长度的序列保留的梯度，这表明序列丢失涉及在细胞系建立的早期阶段从片段的一端删除。我们还利用辐射杂交体为8号染色体的着丝粒周围区域开发了新的序列标记位点。
We have developed a panel of radiation hybrids containing fragments of chromosome 8 as the only human material. The human chromosome content of each cell line was determined relative to an ordered map of sequence tagged sites (STSs) specific to chromosome 8. Between one and four fragments of chromosome 8 were identified in each cell line, with an average of 25% of the STSs retained in each line. Subclones of one radiation hybrid were examined to determine whether all cells within a line are homogeneous with respect to chromosome 8 sequence content. There was considerable variability between subclones, with retention rates for individual STSs ranging from 5 to 100% in different clones. Furthermore, a gradient of retention of sequences along the length of one large chromosome fragment was found, suggesting that sequence loss involved deletions from one end of the fragment at early stages in the establishment of the cell line. We have also made use of the radiation hybrids to develop novel sequence tagged sites for the pericentromeric region of chromosome 8.