Effect of short-term application of nitrogen on the accumulation of β-subunit of β-conglycinin in nitrogen-starved soybean (Glycine max L.) developing seeds
Effect of short-term application of nitrogen on the accumulation of β-subunit of β-conglycinin in nitrogen-starved soybean (Glycine max L.) developing seeds
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短期施氮对缺氮大豆（Glycine max L.）种子发育中β-伴大豆球蛋白β亚基积累的影响
DOI：
10.1080/00380768.2002.10409168
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发表时间：
2002
影响因子：
2
通讯作者：
T. Ohyama
中科院分区：
文献类型：
--
作者：
N. Ohtake;T. Kawachi;I. Okuyama;H. Fujikake;K. Sueyoshi;T. Ohyama
大豆贮藏蛋白β-伴大豆球蛋白β-亚基的积累已有报道。种子，受缺氮抑制。在本报告中，我们试图确定哪种化合物(S)可能在调节完整种子和离体子叶培养体系中β亚单位基因和蛋白质的积累中发挥关键作用。非结瘤大豆植株在缺氮(0.5mMN3−)条件下培养，当植株进入豆荚灌浆期时转移到氮素充足的基质(5mMN3−)中。未成熟种子转入5mMN3-β−培养基后2d内即可检测到DNA亚基及其蛋白的表达。在离体子叶培养体系中，谷氨酰胺能在12h内诱导β亚基的积累，而天冬酰胺在7d内不能诱导β亚基的积累，在天冬酰胺为唯一氮源时，转氨酶抑制剂(氨基氧基乙酸)能促进β亚基的积累而不增加谷氨酰胺的积累。虽然种子中天冬酰胺的浓度反映了整个植株的氮素状况，但未成熟种子中β亚单位基因的积累和蛋白质的持续储存似乎受到谷氨酰胺及其代谢产物或相关化合物的调节。
Abstrct It has been reported that the accumulation of the β-subunit of β-conglycinin, a storage protein of soybean (Glycine max L.) seeds, was suppressed by nitrogen (N)-deficiency. In this report we attempted to determine which compound(s) may playa key role in regulating the accumulation of β-subunit mRNA and protein in intact seeds and in an in vitro cotyledon culture system. Non-nodulating soybean plants were cultivated under N-deficient (0.5 mm NO3 −) conditions, and transferred to N-sufficient medium (5 mm NO3 −) when the plants reached the pod filling stage. The β-subunit mRNA and the protein were detected in immature seeds within 2 d after transfer to 5 mm NO3 − medium. Among the free amino acids in the immature seeds, asparagine concentration increased rapidly within 2 d. In the in vitro cotyledon culture system, the application of glutamine induced the accumulation of β-subunit mRNA within 12 h, while asparagine did not induce the accumulation of β-subunit mRNA for 7 d. An inhibitor of transaminases, (aminooxy)acetic acid, enhanced β-subunit mRNA accumulation without Gln accumulation when asparagine was the sole nitrogen source. Although the asparagine concentration in the seed reflected the nitrogen statues in whole plant, the accumulation of β-subunit mRNA and continuous protein storage in immature seeds appeared to be regulated by glutamine, its metabolites or related compounds.
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs