Talin B is required for force transmission in morphogenesis of Dictyostelium
Talin B is required for force transmission in morphogenesis of Dictyostelium
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DOI：
10.1038/sj.emboj.7600238
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发表时间：
2004-06-02
期刊：
影响因子：
11.4
通讯作者：
Inouye, K
中科院分区：
文献类型：
--
作者：
Tsujioka, M;Yoshida, K;Inouye, K
Talin在局灶性粘连的组装和稳定中发挥着关键作用，但它是否直接参与了形态发生过程中的力传递仍有待阐明。我们发现，无论是在隔离状态下，还是在经历形态发生运动的组织中，突变的Dictyostelials细胞的牵引力都比野生型细胞小得多。突变细胞在紧密堆积的组织中或在体外强抗性条件下的运动能力低于野生型细胞，但在低外力条件下的运动能力并未受到损害，表明突变细胞的力传递效率低下。抗体染色显示Talb基因产物(Talin B)在贴壁部位以小单位形式存在于细胞膜上，不形成大的局部贴壁样集合体。在形态发生过程中，柄前细胞的细胞膜上Talin B的总量比孢子前细胞大。我们的结论是Talin B参与了细胞骨架和细胞外部之间的力传递。
Talin plays a key role in the assembly and stabilisation of focal adhesions, but whether it is directly involved in force transmission during morphogenesis remains to be elucidated. We show that the traction force of Dictyostelium cells mutant for one of its two talin genes talB is considerably smaller than that of wild-type cells, both in isolation and within tissues undergoing morphogenetic movement. The motility of mutant cells in tightly packed tissues in vivo or under strong resistance conditions in vitro was lower than that of wild-type cells, but their motility under low external force conditions was not impaired, indicating inefficient transmission of force in mutant cells. Antibody staining revealed that the talB gene product ( talin B) exists as small units subjacent to the cell membrane at adhesion sites without forming large focal adhesion-like assemblies. The total amount of talin B on the cell membrane was larger in prestalk cells, which exert larger force than prespore cells during morphogenesis. We conclude that talin B is involved in force transmission between the cytoskeleton and cell exterior.