Formation of cartilage in vivo with immobilized autologous rabbit auricular cultured chondrocytes in collagen matrices
Formation of cartilage in vivo with immobilized autologous rabbit auricular cultured chondrocytes in collagen matrices
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DOI：
10.1097/01.prs.0000258849.76630.d2
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发表时间：
2007-05-01
影响因子：
3.6
通讯作者：
Lequerica, Juan L.
中科院分区：
文献类型：
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作者：
Sanz, Ernesto;Penas, Lucas;Lequerica, Juan L.
背景：再生软骨的可用性是重建手术的需求之一。在这项研究中，作者构建了一个由自体固定软骨细胞形成的基质，使用胶原凝胶作为支架。方法：取8只新西兰白色兔耳，取弹性软骨组织，经手术切除，取其组织切片，观察组织学变化。胶原酶11消化软骨后，分离软骨细胞并在培养基中增殖。将软骨细胞固定到牛胶原晶格中并植入，替换去除的天然软骨片。结果：植入软骨细胞的网格形成新的软骨细胞，软骨细胞的数量增加。耳的大体分析显示天然软骨和新软骨在外观、一致性、质地和组织学上的相似性。在新形成的组织中检测到来自双苯甲酰亚胺标记的软骨细胞的细胞核的荧光，指出其在体外培养的起源。在控制或软骨细胞lattices.Conclusion：作者认为，这种方法是未来的临床使用的价值的炎症反应的迹象，可归因于植入物。
Background: The availability of generated cartilage de novo is one of the needs of reconstructive surgery. In this study, the authors constructed a matrix formed by autologous immobilized chondrocytes using collagen gel as a scaffold. Furthermore, the ability of these matrices to engraft and generate new cartilage was examined.Methods: Biopsy specimens of elastic cartilage were surgically obtained from the ears of eight New Zealand White rabbits. After collagenase 11 digestion of cartilage, chondrocytes were isolated and propagated in culture medium. Chondrocytes were immobilized into bovine collagen lattices and implanted, replacing pieces of removed native cartilage. Five weeks after implantation, the rabbits were killed and the ears were examined macroscopically and analyzed by means of histochemical methods.Results: The results show the formation of new cartilage from implanted lattices with chondrocytes. Gross analysis of the ears shows similarities in appearance, consistency, texture, and histology between native and new cartilage. Fluorescence of the nucleus from bisbenzimide-labeled chondrocytes was detected in newly formed tissue, pointing out its in vitro culture origin. No signs of an inflammatory reaction attributable to implants were found in either the control or the chondrocyte lattices.Conclusion: The authors suggest that this approach is of value for future clinical use.