Formation of tubes during self-assembly of bacterial surface layers.
Formation of tubes during self-assembly of bacterial surface layers.
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DOI：
10.1021/la203430q
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发表时间：
2011-11
期刊：
影响因子：
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通讯作者：
M. Bobeth;A. Blecha;A. Blüher;M. Mertig;N. Korkmaz;K. Ostermann;G. Rödel;W. Pompe
中科院分区：
文献类型：
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作者：
M. Bobeth;A. Blecha;A. Blüher;M. Mertig;N. Korkmaz;K. Ostermann;G. Rödel;W. Pompe
在细菌表面(S)层自组装成管的实验研究基础上，提出了描述其基本机理的力学模型，并研究了工艺参数对生长速度和管半径的影响。S层被模拟为具有离散结合位点的曲线板，沿着S层的边缘分布着单体的缔合。已报道的由遗传蛋白质修饰引起的管半径的变化在连续介质力学的框架内被解释。用蒙特卡罗模拟方法分析了S层的生长速度和形状发展与单体在S层的附着和剥离频率的关系。对于弯曲的S层片，导出了形成S层管的判据。因此，只能在初始单体浓度的特定范围内形成管子。此外，还讨论了钙离子浓度对管子形成的影响，包括最近关于钙效应的实验结果。
Based on experimental studies on tube formation during self-assembly of bacterial surface (S)-layers, a mechanistic model for describing the underlying basic mechanisms is proposed and the effect of process parameters on growth velocity and tube radius is investigated. The S-layer is modeled as a curved sheet with discrete binding sites for the association of monomers distributed along the S-layer edges. Reported changes of the tube radius owing to genetic protein modifications are explained within the framework of continuum mechanics. S-layer growth velocity and shape development are analyzed by Monte Carlo simulation in their dependence on the attachment and detachment frequencies of monomers at the S-layer. For curved S-layer patches, a criterion for the formation of S-layer tubes is derived. Accordingly, tubes can form only within a certain range of the initial monomer concentration. Furthermore, the effect of calcium ion concentration on tube formation is discussed, including recent experimental findings on the calcium effect.