Isolation of an anaerobic bacterium which reductively dechlorinates tetrachloroethene and trichloroethene
Isolation of an anaerobic bacterium which reductively dechlorinates tetrachloroethene and trichloroethene
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DOI：
10.1007/bf00115297
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发表时间：
2004
期刊：
影响因子：
3.6
通讯作者：
A. Wild;R. Hermann;T. Leisinger
中科院分区：
文献类型：
--
作者：
A. Wild;R. Hermann;T. Leisinger
TEA菌株是一种严格厌氧的能动杆，具有一到四个侧鞭毛和结晶表面层，从完全将氯化乙烯还原为乙烯的混合培养物中分离出来。该菌以分子氢为电子供体，将四氯乙烯或三氯乙烯还原脱卤生成顺式-1，2-二氯乙烯。它是无法生长发酵或在存在的三或四氯乙烯与葡萄糖，丙酮酸，乳酸，乙酸或甲酸。16 SrDNA序列分析表明，TEA菌株与限制性脱卤菌的16 SrDNA序列同源性为99.7%。因此，这两种微生物是革兰氏阳性菌芽孢杆菌/梭菌亚门内相同种或相同属的代表。
Strain TEA, a strictly anaerobic, motile rod with one to four lateral flagella and a crystalline surface layer was isolated from a mixed culture that completely reduces chlorinated ethenes to ethene. The organism coupled reductive dehalogenation of tetrachloroethene or trichloroethene tocis-1,2-dichloroethene to growth, using molecular hydrogen as the electron donor. It was unable to grow fermentatively or in the presence of tri- or tetrachloroethene with glucose, pyruvate, lactate, acetate or formate. The 16S rDNA sequence of strain TEA was 99.7% identical to that ofDehalobacter restrictus. The two organisms thus are representatives of the same species or the same genus within theBacillus/Clostridiumsubphylum of the gram-positive bacteria.