Optimizing HAPEX™ topography influences osteoblast response
Optimizing HAPEX™ topography influences osteoblast response
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DOI：
10.1089/107632702760184718
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发表时间：
2002-06-01
影响因子：
--
通讯作者：
Bonfield, W
中科院分区：
文献类型：
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作者：
Dalby, MJ;Di Silvio, L;Bonfield, W
HAPEX（TM）（羟基磷灰石增强聚乙烯复合材料）是第二代骨科生物材料，设计为骨模拟材料，已获得临床成功。在细胞工程中使用拓扑结构已被证明会影响细胞附着和随后的反应。因此，通过结合生物活性和增强成骨细胞对植入物表面的反应，可以实现改善的组织修复和植入物寿命。在这项研究中，原代人成骨细胞样细胞模型已被用于研究的表面形貌和化学的影响，由三种不同的生产方法。扫描电子显微镜，荧光显微镜，共聚焦扫描激光显微镜已被用来研究细胞粘附;氚标记的胸苷摄取已被用来观察细胞增殖;和逆转录酶-聚合酶链反应和生化方法已被用来研究表型表达。透射电子显微镜也被用来观察更长期的形态。结果表明，地形显着影响细胞反应，并可能是一种手段，提高骨附着在HAPEX（TM）。
HAPEX(TM) (hydroxyapatite-reinforeed polyethylene composite) is a second-generation orthopedic biomaterial designed as a bone analog material, which has found clinical success. The use of topography in cell engineering has been shown to affect cell attachment and subsequent response. Thus, by combining bioactivity and enhancing osteoblast response to the implant surface, improved tissue repair and implant life span may be achieved. In this study a primary human osteoblast-like cell model has been used to study the influence of surface topography and chemistry produced by three different production methods. Scanning electron microscopy, fluorescence microscopy, and confocal scanning laser microscopy have been used to study cell adhesion; tritiated thymidine uptake has been used to observe cell proliferation; and the reverse transcriptase-polymerase chain reaction and biochemical methods have been used to study phenotypic expression. Transmission electron microscopy has also been used to look at more long-term morphology. The results show that topography significantly influences cell response, and may be a means of enhancing bone apposition on HAPEX(TM).