2 HOMOLOGOUS ZINC-FINGER GENES IDENTIFIED BY MULTICOPY SUPPRESSION IN A SNF1 PROTEIN-KINASE MUTANT OF SACCHAROMYCES-CEREVISIAE
2 HOMOLOGOUS ZINC-FINGER GENES IDENTIFIED BY MULTICOPY SUPPRESSION IN A SNF1 PROTEIN-KINASE MUTANT OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.13.7.3872
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发表时间：
1993-07-01
影响因子：
5.3
通讯作者：
CARLSON, M
中科院分区：
文献类型：
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作者：
ESTRUCH, F;CARLSON, M
MSN2 基因被选为酿酒酵母温度敏感 SNF1 蛋白激酶突变体的多拷贝抑制基因。 MSN2 编码与酵母 MIG1 阻遏物、哺乳动物早期生长反应和肾母细胞瘤锌指蛋白相关的 Cys2His2 锌指蛋白。 MSN2 的缺失不会引起表型。第二个相似的锌指基因 MSN4 被分离出来，这两个基因的缺失会导致与碳利用相关的表型缺陷。锌指区域的过度表达对生长有害。 LexA-MSN2 和 LexA-MSN4 融合蛋白与 DNA 结合时可充当强转录激活剂。讨论了该锌指蛋白家族的功能作用。
The MSN2 gene was selected as a multicopy suppressor in a temperature-sensitive SNF1 protein kinase mutant of Saccharomyces cerevisiae. MSN2 encodes a Cys2His2 zinc finger protein related to the yeast MIG1 repressor and to mammalian early growth response and Wilms' tumor zinc finger proteins. Deletion of MSN2 caused no phenotype. A second similar zinc finger gene, MSN4, was isolated, and deletion of both genes caused phenotypic defects related to carbon utilization. Overexpression of the zinc finger regions was deleterious to growth. LexA-MSN2 and LexA-MSN4 fusion proteins functioned as strong transcriptional activators when bound to DNA. Functional roles of this zinc finger protein family are discussed.