Scanometric DNA array detection with nanoparticle probes
Scanometric DNA array detection with nanoparticle probes
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DOI：
10.1126/science.289.5485.1757
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发表时间：
2000-09-08
期刊：
影响因子：
56.9
通讯作者：
Letsinger, RL
中科院分区：
文献类型：
--
作者：
Taton, TA;Mirkin, CA;Letsinger, RL
本文描述了一种使用荧光素修饰的金纳米粒子探针和传统的平板扫描仪分析组合DNA阵列的方法。用纳米颗粒而不是荧光团探针标记寡核苷酸靶标基本上改变了来自阵列基底的靶标的解链概况。这种差异允许从具有单核苷酸错配的靶标中区分寡核苷酸序列，其选择性是对荧光团标记的靶标观察到的选择性的三倍以上。此外，当与基于纳米颗粒促进的银（I）还原的信号放大方法结合时，该扫描阵列检测系统的灵敏度超过类似荧光团系统两个数量级。
A method for analyzing combinatorial DNA arrays using oligonucleotide-modified gold nanoparticle probes and a conventional flatbed scanner is described here. Labeling oligonucleotide targets with nanoparticle rather than fluorophore probes substantially alters the melting profiles of the target; from an array substrate. This difference permits the discrimination of an oligonucleotide sequence from targets with single nucleotide mismatches with a selectivity that is over three times that observed for fluorophore-labeled targets. In addition, when coupled with a signal amplification method based on nanoparticle-promoted reduction of silver(I), the sensitivity of this scanometric array detection system exceeds that of the analogous fluorophore system by two orders of magnitude.