Effect of 8-week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats.
Effect of 8-week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats.
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DOI：
10.14814/phy2.13725
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发表时间：
2018-06
影响因子：
2.5
通讯作者：
Kim CK
中科院分区：
文献类型：
--
作者：
Lim CH;Gil JH;Quan H;Viet DH;Kim CK
我们研究了在给予不同剂量的亮氨酸后，常规亮氨酸摄入和/或抗阻运动训练对骨骼肌肥大和卫星细胞活性的影响。将10周龄的Sprague-Dawley大鼠分为6组（每组n = 7）：对照组（Con），两组接受10%（0.135 g/kg.wt）（Leu 10）或50%（0.675 g/kg.wt）（Leu 50）亮氨酸补充，三个运动组接受0%（Ex），10%（Leu 10 Ex）和50%（Leu 50 Ex）亮氨酸补充。大鼠每周进行三次爬梯运动，持续8周，并每天在同一时间接受亮氨酸补充剂。通过免疫组织化学评估肌肉表型。通过蛋白质印迹法测定MyoD、肌细胞生成素和IGF 1蛋白水平。Leu 50 Ex组的阳性胚胎肌球蛋白纤维（0.35 ± 0.08，250%）和肌核（3.29 ± 0.3，118.7%）数量显著高于其他组。运动训练组的肌横截面积、肌卫星细胞数及MyoD、myogenin和IGF 1 α蛋白表达均较对照组增加（P < 0.05）。而单纯添加亮氨酸组无论在何种剂量下均未增加骨骼肌肥大和卫星细胞活性（P > 0.05）。亮氨酸摄入伴随着定期运动训练可能会增加骨骼肌中卫星细胞的激活，并比单独持续摄入亮氨酸更有效地改善肌肉质量。
We investigated the effects of regular leucine intake and/or resistance exercise training on skeletal muscle hypertrophy and satellite cell activity after the administration of different doses of leucine. Ten‐week‐old Sprague–Dawley rats were assigned to six groups (n = 7 per group): a control group (Con), two groups receiving either 10% (0.135 g/kg.wt) (Leu10) or 50% (0.675 g/kg.wt) (Leu50) leucine supplementation, and three exercise groups receiving 0% (Ex), 10% (Leu10Ex), and 50% (Leu50Ex) leucine supplementation. The rats performed ladder climbing exercises thrice per week for 8 weeks, and received leucine supplements at the same time daily. Muscle phenotypes were assessed by immunohistochemistry. MyoD, myogenin, and IGF1 protein levels were determined by western blot. The Leu50Ex group displayed significantly higher numbers of positive embryonic myosin fibers (0.35 ± 0.08, 250%) and myonuclei (3.29 ± 0.3, 118.7%) than all other groups. And exercise training groups increased the cross‐sectional area, the number of satellite cells and protein expression of MyoD, myogenin, and IGF1alpha relative to the Control group (P < 0.05). However, Only leucine supplementation group did not increase skeletal muscle hypertrophy and satellite cell activity, regardless of the dose (P > 0.05). Leucine intake accompanied by regular exercise training may increase satellite cell activation in skeletal muscles, and improve muscle quality more effectively than continuous leucine ingestion alone.