Stimulation of lymphocyte proliferation by oyster glycogen sulfated at C-6 position
Stimulation of lymphocyte proliferation by oyster glycogen sulfated at C-6 position
复制标题
C-6 位硫酸化牡蛎糖原刺激淋巴细胞增殖
DOI：
10.1016/j.carbpol.2013.01.057
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发表时间：
2013-04-15
影响因子：
11.2
通讯作者：
Yu, Chenxu
中科院分区：
文献类型：
--
作者：
Yang, Jingfeng;Zhu, Beiwei;Yu, Chenxu
本研究以大连湾牡蛎为原料，提取糖原，并以糖原为模型物质，研究多糖的构效关系。采用甲基化分析、核磁共振（NMR）和红外光谱（IR）对纯化的牡蛎糖原进行了表征。采用氯磺酸-吡啶法对牡蛎糖原进行硫酸酯化，并通过13 C NMR谱鉴定了一种C-6取代的物种（SOG）为主要的硫酸酯化物种。SOG的分子量和硫酸根含量分别测定为3.2 × 10（4）g/mol和33.6%。另一种具有C-2和C-3取代的硫酸化牡蛎糖原物质（SOG 1）也在最终产品中以较低的量被鉴定。SOG在体外对脾淋巴细胞增殖的刺激作用明显强于SOG 1，表明硫酸化糖原的取代位置是影响其刺激淋巴细胞增殖的主要因素。(C)2013爱思唯尔有限公司保留所有权利。
In this study, glycogen was extracted from oyster Ostrea talienwhanensis Crosse and used as a model to investigate the structure-activity correlation of polysaccharides. Purified oyster glycogen was characterized by methylation analysis, nuclear magnetic resonance (NMR) spectroscopy and infrared spectroscopy (IR). The oyster glycogen was subsequently sulfated by chlorosulfonic acid-pyridine method, and a C-6 substituted species (SOG) was identified to be the primary sulfated oyster glycogen species by C-13 NMR spectroscopy. The molecular weight and sulfate content of the SOG was determined to be 3.2 x 10(4) g/mol and 33.6%, respectively. Another sulfated oyster glycogen species (SOG1) with C-2 and C-3 substitution was also identified at a lesser amount in the final product. SOG exhibited a much stronger stimulation effect to splenic lymphocyte proliferation than SOG1 in vitro, indicating that the position of sulfate substitution is a major determining factor on the efficacy of sulfated glycogens to stimulate lymphocyte proliferation. (C) 2013 Elsevier Ltd. All rights reserved.