Evaluating the challenges and reproducibility of studies investigating DNA methylation signatures of psychological stress.
Evaluating the challenges and reproducibility of studies investigating DNA methylation signatures of psychological stress.
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DOI：
10.2217/epi-2021-0190
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发表时间：
2022-02
期刊：
影响因子：
3.8
通讯作者：
Yun Zhang;Chunyu Liu
中科院分区：
文献类型：
--
作者：
Yun Zhang;Chunyu Liu
心理压力会增加各种负面健康结果的风险。已经完成了一些研究，以确定人类大脑中的DNA甲基化变化是否因压力而发生，并与长期影响和疾病有关，但结果一直不一致。对人类候选基因研究（150）和表观基因组关联研究（68）进行了系统评估，以评估DNA甲基化如何受到产前、幼儿和成年期压力的影响。NR3C1外显子1F的DNA甲基化与儿童虐待和早期生活逆境之间的关联得到了很好的证明，但其他基因没有表现出明确的关联。表观基因组关联研究中单个CpG位点的重复性也很差。然而，生物学途径，包括压力反应，大脑发育和免疫力，在整个生命周期中一直在不同的压力源中被确定。未来的研究将受益于样本量的增加，纵向设计，标准化的方法，最佳的质量控制，和改进的统计程序。
Psychological stress can increase the risk of a wide range of negative health outcomes. Studies have been completed to determine if DNA methylation changes occur in the human brain because of stress and are associated with long-term effects and disease, but results have been inconsistent. Human candidate gene studies (150) and epigenome-wide association studies (68) were systematically evaluated to assess how DNA methylation is impacted by stress during the prenatal period, early childhood and adulthood. The association between DNA methylation of NR3C1 exon 1F and child maltreatment and early life adversity was well demonstrated, but other genes did not exhibit a clear association. The reproducibility of individual CpG sites in epigenome-wide association studies was also poor. However, biological pathways, including stress response, brain development and immunity, have been consistently identified across different stressors throughout the life span. Future studies would benefit from the increased sample size, longitudinal design, standardized methodology, optimal quality control, and improved statistical procedures.