ARE THE POLARIZATION COLORS OF PICROSIRIUS RED-STAINED COLLAGEN DETERMINED ONLY BY THE DIAMETER OF THE FIBERS
ARE THE POLARIZATION COLORS OF PICROSIRIUS RED-STAINED COLLAGEN DETERMINED ONLY BY THE DIAMETER OF THE FIBERS
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DOI：
10.1007/bf00266843
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发表时间：
1989-01-01
期刊：
影响因子：
--
通讯作者：
WOLMAN, M
中科院分区：
文献类型：
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作者：
DAYAN, D;HISS, Y;WOLMAN, M
在相同厚度的纤维中，研究了不同纯化胶原蛋白的偏振颜色。将三种不同的可溶性I型胶原，不溶性I型胶原，甲状旁腺胶原I型，两种p-N-I型胶原，胃酶提取物胶原II，两种可溶性III型胶原，对N-III型胶原和V型可溶性胶原进行常规的组织病理学固定，制备5-µm厚的切片，用小天狼星红染色，并在交叉极下观察。测定了细纤维(0.8µm或更小)和粗纤维(1.6-2.4µm)的偏振颜色。胶原蛋白和p-N-胶原蛋白的大部分细纤维呈绿色到黄绿色的偏振颜色，不同样品之间没有明显差异。所有p-N-胶原、中性胶原和正常0.15 M氯化钠可溶性胶原的粗大纤维均呈绿色至绿黄偏振色，而在所有其他胶原中，均可观察到波长较长的偏振色(从黄橙至红色)。这些数据表明，纤维厚度并不是决定红天狼星红染胶原蛋白偏振颜色的唯一因素。紧密排列的胶原蛋白分子可能排列得更好，显示出波长更长的偏振颜色。因此，胶原分子的堆积不仅对纤维厚度起作用，而且对红星红染胶原色的偏振模式也有影响。
Polarization colors of various purified collagens were studied in fibers of similar thickness. Three different soluble collagens of type I, insoluble collagen type I, lathyritic collagen type I, two p-N-collagens type I, pepsin extract collagen type II, two soluble collagens type III, p-N-collagen type III, and soluble collagen type V were submitted to a routine histopathologic procedure of fixation, preparation of 5-.mu.m-thick sections, staining with Picrosirius red and examination under crossed polars. Polarization colors were determined for thin fibers (0.8 .mu.m or less) and thick fibers, (1.6-2.4 .mu.m). Most thin fibers of collagens and p-N-collagens showed green to yellowish-green polarization colors with no marked differences between the various samples. Thick fibers of all p-N-collagens, lathyritic and normal 0.15 M NaCl-soluble collagens showed green to greenish-yellow polarization colors, while in all other collagens, polarization colors of longer wavelengths (from yellowish-orange to red) were observed. These data suggested that fiber thickness was not the only factor involved in determining the polarization colors of Picrosirius red-stained collagens. Tightly packed and presumably, better aligned collagen molecules showed polarization colors of longer wavelengths. Thus, packing of collagen molecules and not only fiber thickness plays a role in the pattern of polarization colors of Picrosirius red-stained collagens.