Presence of Porphyromonas gingivalis in esophagus and its association with the clinicopathological characteristics and survival in patients with esophageal cancer.
Presence of Porphyromonas gingivalis in esophagus and its association with the clinicopathological characteristics and survival in patients with esophageal cancer.
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DOI：
10.1186/s13027-016-0049-x
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发表时间：
2016
影响因子：
3.7
通讯作者：
Feng X
中科院分区：
文献类型：
--
作者：
Gao S;Li S;Ma Z;Liang S;Shan T;Zhang M;Zhu X;Zhang P;Liu G;Zhou F;Yuan X;Jia R;Potempa J;Scott DA;Lamont RJ;Wang H;Feng X
越来越多的证据表明特定细菌感染与某些恶性肿瘤的发生之间存在因果关系。然而，关键的牙周病原体牙龈卟啉单胞菌在食管鳞状细胞癌（ESCC）中可能扮演的角色仍然未知。因此，我们检测了牙龈卟啉单胞菌在食管黏膜中的存在情况，以及牙龈卟啉单胞菌感染与ESCC的诊断和预后之间的关系。
使用针对全菌及其独特分泌的蛋白酶——牙龈蛋白酶Kgp的抗体，通过免疫组织化学方法检测ESCC患者和正常对照者食管组织中牙龈卟啉单胞菌的存在情况。实时定量聚合酶链反应（qRT - PCR）被用作一种验证方法来检测牙龈卟啉单胞菌的16S rDNA。收集临床病理特征以分析牙龈卟啉单胞菌感染与ESCC发生之间的关系。
通过免疫组织化学方法，在61%的癌组织、12%的癌旁组织中检测到牙龈卟啉单胞菌，而在正常食管黏膜中未检测到。还观察到赖氨酸特异性牙龈蛋白酶（一种由牙龈卟啉单胞菌独特分泌的催化性内切蛋白酶）以及牙龈卟啉单胞菌16S rDNA有类似的分布情况。此外，统计相关性表明牙龈卟啉单胞菌感染与多种临床病理特征呈正相关，包括分化状态、转移以及总生存率。
这些发现首次证明牙龈卟啉单胞菌感染ESCC患者的食管上皮，建立了牙龈卟啉单胞菌感染与ESCC进展之间的关联，并提示牙龈卟啉单胞菌感染可能是这种疾病的一个生物标志物。更重要的是，如果这些数据得到证实，表明根除一种常见的口腔病原体可能有助于减轻ESCC的总体负担。
本文的网络版（doi:10.1186/s13027 - 016 - 0049 - x）包含补充材料，可供授权用户使用。
Mounting evidence suggests a causal relationship between specific bacterial infections and the development of certain malignancies. However, the possible role of the keystone periodontal pathogen, Porphyromonas gingivalis, in esophageal squamous cell carcinoma (ESCC) remains unknown. Therefore, we examined the presence of P. gingivalis in esophageal mucosa, and the relationship between P. gingivalis infection and the diagnosis and prognosis of ESCC. The presence of P. gingivalis in the esophageal tissues from ESCC patients and normal controls was examined by immunohistochemistry using antibodies targeting whole bacteria and its unique secreted protease, the gingipain Kgp. qRT-PCR was used as a confirmatory approach to detect P. gingivalis 16S rDNA. Clinicopathologic characteristics were collected to analyze the relationship between P. gingivalis infection and development of ESCC. P. gingivalis was detected immunohistochemically in 61 % of cancerous tissues, 12 % of adjacent tissues and was undetected in normal esophageal mucosa. A similar distribution of lysine-specific gingipain, a catalytic endoprotease uniquely secreted by P. gingivalis, and P. gingivalis 16S rDNA was also observed. Moreover, statistic correlations showed P. gingivalis infection was positively associated with multiple clinicopathologic characteristics, including differentiation status, metastasis, and overall survival rate. These findings demonstrate for the first time that P. gingivalis infects the epithelium of the esophagus of ESCC patients, establish an association between infection with P. gingivalis and the progression of ESCC, and suggest P. gingivalis infection could be a biomarker for this disease. More importantly, these data, if confirmed, indicate that eradication of a common oral pathogen could potentially contribute to a reduction in the overall ESCC burden. The online version of this article (doi:10.1186/s13027-016-0049-x) contains supplementary material, which is available to authorized users.