GRANULE CELL GENESIS IN THE HIPPOCAMPUS OF RATS TREATED NEONATALLY WITH HYDROCORTISONE
GRANULE CELL GENESIS IN THE HIPPOCAMPUS OF RATS TREATED NEONATALLY WITH HYDROCORTISONE
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DOI：
10.1016/0306-4522(80)90045-7
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发表时间：
1980-01-01
期刊：
影响因子：
3.3
通讯作者：
BOHN, MC
中科院分区：
文献类型：
--
作者：
BOHN, MC
大鼠出生后第1~4天注射醋酸氢化可的松，用[~3H]胸腺嘧啶核苷放射自显影技术追踪齿状回颗粒细胞的发生。在短期存活的放射自显影实验中，在氢化可的松治疗过程中和治疗后即刻，[~3H]胸腺嘧啶核苷标记的细胞在齿状门区和颗粒层中的数量减少，但在第2周恢复到对照水平。这一区域的生长缓慢，表现为第7天颗粒层体积减少20%，第4-6天整个海马区DNA含量减少14%。60d时，治疗组大鼠颗粒层体积与对照组相比无显著差异。用长寿命放射自显影技术观察治疗组和对照组大鼠海马背侧和腹侧的颗粒细胞生日。在背部，出生后3周以上的颗粒细胞的出生模式没有显著的影响。处理组大鼠腹侧、颗粒细胞的发生在第5天明显受到抑制，而对照组的抑制作用最大。这将颗粒细胞在这个水平上的生日高峰推迟了几天。新生儿期糖皮质激素治疗可明显改变大鼠海马生后颗粒细胞发生的速度和方式。将糖皮质激素治疗的大鼠小脑神经发生的具体效果与先前报道的结果进行了比较。
Rats were injected with hydrocortisone acetate on postnatal days 1-4 and the genesis of granule cells in the dentate gyrus was followed by autoradiographic techniques using [3H]thymidine. In short-survival autoradiographic experiments, the number of cells labeled by [3H]thymidine throughout the dentate hilus and stratum granulosum was decreased during and immediately after hydrocortisone treatment but recovered to control values during the 2nd wk. Growth of this region was retarded as indicated by a 20% reduction in volume of the stratum granulosum at 7 days and 14% deficit in DNA content in the whole hippocampus on days 4-6. At 60 days, the volume of the stratum granulosum in treated rats was not significantly different from that of controls. Granule cell birthdays at dorsal and ventral levels of the hippocampus were investigated by long-survival autoradiography in treated and control groups. Dorsally, the pattern of granule cell birthdays over the 1st 3 postnatal wk was not significantly affected. Ventrally, granule cell genesis in treated rats was significantly depressed on day 5 when that in controls was maximal. This delayed the peak of granule cell birthdays at this level by several days. The rate and pattern of postnatal granule cell genesis in the rat hippocampus are apparently altered by neonatal glucocorticoid treatment. Specific effects are compared to those previously reported for cerebellar neurogenesis in the glucocorticoid-treated rat.