Novel mechanism of metabolic co-regulation coordinates the biosynthesis of secondary metabolites in Pseudomonas protegens
Novel mechanism of metabolic co-regulation coordinates the biosynthesis of secondary metabolites in Pseudomonas protegens
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DOI：
10.7554/elife.22835
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发表时间：
2017-03
期刊：
影响因子：
7.7
通讯作者：
Qing Yan;B. Philmus;Jeff H. Chang;J. Loper
中科院分区：
文献类型：
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作者：
Qing Yan;B. Philmus;Jeff H. Chang;J. Loper
次生代谢物的生物合成途径之间的代谢协同调节在微生物中很常见，并在微生物相互作用中发挥重要作用。在这里，我们描述了一种新的代谢共调节机制，其中一种途径中的中间体转化为激活另一种途径的信号。我们的研究重点是土壤细菌假单胞菌蛋白酶产生的两种抗菌代谢物2,4-二乙酰基间苯三酚（DAPG）和pyoluteorin的共调控。我们发现，在DAPG生物合成中的中间产物间苯三酚被pyoluteorin基因簇中编码的卤化酶转化为单氯和二氯间苯三酚。氯代间苯三酚作为细胞内和细胞间信号，诱导绿脓毒苷生物合成基因的表达、绿脓毒苷的产生以及绿脓毒苷介导的对植物致病菌淀粉Erwinia amylovora的抑制。这种代谢协同调节为P. protegens优化在合作和竞争微生物相互作用中具有不同作用的次级代谢物的部署提供了一种策略。DOI: http://dx.doi.org/10.7554/eLife.22835.001
Metabolic co-regulation between biosynthetic pathways for secondary metabolites is common in microbes and can play an important role in microbial interactions. Here, we describe a novel mechanism of metabolic co-regulation in which an intermediate in one pathway is converted into signals that activate a second pathway. Our study focused on the co-regulation of 2,4-diacetylphloroglucinol (DAPG) and pyoluteorin, two antimicrobial metabolites produced by the soil bacterium Pseudomonas protegens. We show that an intermediate in DAPG biosynthesis, phloroglucinol, is transformed by a halogenase encoded in the pyoluteorin gene cluster into mono- and di-chlorinated phloroglucinols. The chlorinated phloroglucinols function as intra- and inter-cellular signals that induce the expression of pyoluteorin biosynthetic genes, pyoluteorin production, and pyoluteorin-mediated inhibition of the plant-pathogenic bacterium Erwinia amylovora. This metabolic co-regulation provides a strategy for P. protegens to optimize the deployment of secondary metabolites with distinct roles in cooperative and competitive microbial interactions. DOI: http://dx.doi.org/10.7554/eLife.22835.001