Dynamic transcriptome response in Meretrix meretrix to Aroclor 1254 exposure
Dynamic transcriptome response in Meretrix meretrix to Aroclor 1254 exposure
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DOI：
10.1016/j.ecoenv.2020.111485
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发表时间：
2021-01-01
影响因子：
6.8
通讯作者：
Cai，Yuefeng
中科院分区：
文献类型：
--
作者：
Li，Yongqi;Liu，Shishi;Cai，Yuefeng
多氯联苯(PCbs)是众所周知的持久性有机污染物，通过改变人体新陈代谢对免疫系统、生殖系统和内分泌系统产生毒害作用。为了阐明其潜在的分子机制，文昌鱼暴露于10和1000 ng/L Aroclor1254和天然海水(对照)中。分别采集暴露于天然海水和Aroclor 1254暴露1天和3天的文蛤样本进行转录组分析。组装后，获得了超过535,157个转录本，平均长度为949个碱基对，N50长度为1279个碱基；最终产生了177,142个非基因。在本研究中，共鉴定出5101个差异表达基因。差异表达的基因与解毒代谢、氧化应激、免疫反应和内分泌系统紊乱有关。在这些基因中，细胞色素P45020A1(2.06~4.46倍)、谷胱甘肽S转移酶(2.25~3.80倍)、多药耐药相关蛋白1(1.49~2.92倍)、过氧化物酶样蛋白(1.33~4.26倍)、溶菌酶(1.61~2.05倍)、bcl2样蛋白(1.14~2.29倍)和卵黄蛋白原(1.09~1.19倍)均显著诱导表达。同时，细胞色素P450 2J5(−1.20~−2.86倍)、细胞色素P450 3A24(−1.40~−4.08倍)、C1q(−1.27~−1.66倍)、磺基转移酶(−1.51~−1.84倍)、单羧酸转运蛋白10(−1.30~−4.70倍)、3-β羟基类固醇脱氢酶(−1.43~−2.81倍)和β-半乳糖苷酶(−1.23~−2.23倍)等基因表达下调。细胞色素P450 2 J5、谷胱甘肽S转移酶、3β羟基类固醇脱氢酶和β半乳糖苷酶的表达水平具有时间效应和剂量效应。本研究提供了对Aroclor 1254暴露于INM的毒性影响的见解。梅雷特里克斯。
Polychlorinated biphenyls (PCBs) are well-known persistent organic pollutants; they have toxic effects on the immune system, reproductive system, and endocrine system by changing the metabolism of the body. To elucidate the underlying molecular mechanism, the clamMeretrix meretrixwas exposed to 10 and 1000 ng/L Aroclor 1254 and natural seawater (control). Samples from clams exposed to natural seawater and those exposed to Aroclor 1254 for 1 and 3 days were individually collected for transcriptome analysis. After assembly, more than 535,157 transcripts with a mean length of 949 bp and an N50 length of 1279 bp were obtained; a final set of 177,142 unigenes was generated. In the present study, 5101 differentially expressed genes were identified. The differentially expressed genes were related to detoxification metabolism, oxidative stress, immune response, and endocrine system disruption. Of these genes, under the Aroclor 1254 exposure, cytochrome P450 20A1 (2.06–4.46 folds), glutathione S-transferase (2.25–3.80 folds), multidrug resistance-associated protein 1-like (1.49–2.92 folds), peroxidase-like protein (1.33–4.26 folds), lysozyme (1.61–2.05 folds), bcl-2 like 1 protein (1.14–2.29 folds) and vitellogenin (1.09–1.19 folds) showed been significantly induced expressed. At the same time, some genes were down regulated, including cytochrome P450 2J5 (−1.20 ~ −2.86 folds), cytochrome P450 3A24 (−1.40 ~ −4.08 folds), C1q (−1.27 ~ −1.66 folds), Sulfotransferase (−1.51 ~ −1.84 folds), monocarboxylate transporter 10 (−1.30 ~ −4.70 folds), 3-beta hydroxysteroid dehydrogenase (−1.43 ~ −2.81 folds) and beta-galactosidase (−1.23 ~ −2.23 folds). Furthermore, it showed that the expression levels of CYP2J5, glutathione S-transferase, 3-beta hydroxysteroid dehydrogenase and beta-galactosidase had time responses and dose responses. The present study provided insights into the toxic effects of Aroclor 1254 exposure inM. meretrix.