ELECTRON MICROSCOPY OF NANOPLANKTON FROM THE NORTH PACIFIC CENTRAL GYRE 1
ELECTRON MICROSCOPY OF NANOPLANKTON FROM THE NORTH PACIFIC CENTRAL GYRE 1
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北太平洋中央环流纳米浮游生物的电子显微镜 1
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
L. Haas
中科院分区：
文献类型：
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作者：
N. Hoepffner;L. Haas
纳米浮游生物是1985年8月在“梅尔维尔”号R/V号游轮上从北太平洋中央环流采集的。取表层和深层叶绿素最大层的水样用戊二醛固定，并在Formvar涂覆的网格上进行电子显微镜观察。首先在蓝光下用荧光显微镜观察网格，以区分含有叶绿体和非叶绿体的细胞。表层和深层样品中以裸藻属占优势，占已鉴定生物总数的55%。Pymnesiales和Coccosphaerales同样丰富，尽管在深的叶绿素最大层中，带有球藻的细胞往往占主导地位。异养尾鞭毛虫在深海中也很丰富，具有很高的物种多样性。普氏微藻(Micromonas pusilla(Butcher)Manton&Parke)是绿藻纲的主要代表，常见于深部叶绿素高值区。我们的观察揭示了几个新物种，包括自养和异养标本，并证明了了解浮游群落结构对生态学的重要性。事实上，深海中大量异养生物的存在表明了一条活跃的微生物食物链，这可能在调节少营养水域的浮游生物过程中发挥重要作用。
Nanoplankton was collected from the North Pacific Central Gyre during a cruise of R/V “Melville” in August 1985. Water samples from the surface and the deep chlorophyll maximum layer were fixed with glutaraldehyde and prepared for electron microscopy observation using formvar coated grids. The grids were examined first by epifluoresence microscopy under blue light to differentiate chloroplast and non‐chloroplast containing cells. The surface and deep samples were dominated strongly by members of the Prymnesiophyceae, accounting for 55% of the total number of organisms identified. Prymnesiales and Coccosphaerales were equally abundant although coccoliths‐bearing cells tended to dominate in the deep chlorophyll maximum layer. Heterotrophic choanoflagellates were also abundant at depth and were characterized by a high species diversity. Micromonas pusilla (Butcher) Manton & Parke was the major representative of the Prasinophyceae and was observed commonly within the deep chlorophyll maximum. Our observations reveal several new species, including autotrophic and heterotrophic specimens, and demonstrate the importance of knowing the structure of the planktonic community for ecological purposes. Indeed, the presence of a large number of heterotrophic organisms in the deep water suggests an active microbial food chain which may play an important role in regulating plankton processes in oligotrophic waters.