Corneal endothelium bicarbonate transport and the effect of carbonic anhydrase inhibitors on endothelial permeability and fluxes and corneal thickness.
Corneal endothelium bicarbonate transport and the effect of carbonic anhydrase inhibitors on endothelial permeability and fluxes and corneal thickness.
复制标题
角膜内皮碳酸氢盐转运以及碳酸酐酶抑制剂对内皮通透性和通量以及角膜厚度的影响。
DOI：
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发表时间：
1977
影响因子：
4.4
通讯作者：
H. Wynn
中科院分区：
文献类型：
--
作者：
D. S. Hull;K. Green;M. Boyd;H. Wynn
测定了碳酸氢盐穿过角膜内皮的单向通量。证明了碳酸氢盐从基质到面向水的表面的净通量。在碳酸酐酶抑制的条件下，净碳酸氢盐通量减少了 54%，并且内皮的被动水渗透性增加。镜面显微镜实验表明，在碳酸酐酶抑制条件下，角膜肿胀率增加。将 H+ 识别为与碳酸氢根离子一起移动以维持电中性的阳离子的尝试并未成功，因为不可能维持内皮细胞上不同的 H+ 浓度差。
Unidirectional fluxes of bicarbonate across the corneal endothelium were determined. A net flux of bicarbonate from the stromal to aqueous-facing surface was demonstrated. Under conditions of carbonic anhydrase inhibition the net bicarbonate flux was reduced by 54 percent and the passive water permeability of the endothelium was increased. Experiments with the specular microscope demonstrated an increased rate of corneal swelling under conditions of carbonic anhydrase inhibition. Attempts to identify H+ as the cation moving with the bicarbonate ion to maintain electroneutrality were not successful because of the impossibility of sustaining a differential H+ concentration difference across the endothelium.