Approach to the Investigation of Nucleosome Structure by Using the Highly Emissive Nucleobase thdG-tC FRET Pair
Approach to the Investigation of Nucleosome Structure by Using the Highly Emissive Nucleobase thdG-tC FRET Pair
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DOI：
10.1002/chem.201803382
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发表时间：
2018-11-16
影响因子：
4.3
通讯作者：
Sugiyama，Hiroshi
中科院分区：
文献类型：
--
作者：
Han，Ji Hoon;Park，Soyoung;Sugiyama，Hiroshi
依赖于距离和方向因子的FRET系统是研究核小体构象动力学的一种有用和有吸引力的方法。在这项研究中，应用高发射nucleobasethdG-tC FRET对601核小体的报告。 结果发现，第三个dG-tC FRET对成功地掺入到145 bp的601序列，并获得不同的FRET效率为指定的供体和受体的位置在核小体。
A distance‐ and orientation‐factor‐dependent FRET system is a useful and attractive approach to the investigation of the conformational dynamics of nucleosomes. In this study, the application of the highly emissive nucleobasethdG–tC FRET pair to 601 nucleosomes is reported. It was found that thethdG–tC FRET pair was successfully incorporated to 145 bp 601 sequences, and different FRET efficiencies were obtained for the designated donor and acceptor positions in the nucleosome.