Arabidopsis Flower and Embryo Developmental Genes are Repressed in Seedlings by Different Combinations of Polycomb Group Proteins in Association with Distinct Sets of Cis-regulatory Elements.
Arabidopsis Flower and Embryo Developmental Genes are Repressed in Seedlings by Different Combinations of Polycomb Group Proteins in Association with Distinct Sets of Cis-regulatory Elements.
复制标题
拟南芥花和胚胎发育基因在幼苗中受到与不同组顺式调控元件相关的多梳蛋白的不同组合的抑制
DOI：
10.1371/journal.pgen.1005771
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发表时间：
2016-01
期刊：
影响因子：
4.5
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Wang H;Liu C;Cheng J;Liu J;Zhang L;He C;Shen WH;Jin H;Xu L;Zhang Y
多梳抑制复合物（PRCs）在植物和动物的转录抑制和发育调控中起着至关重要的作用。在植物中，PRCs不同成员的缺失导致重叠和独特的表型缺陷。然而，决定靶特异性和功能多样性的潜在分子机制尚未得到充分表征。在这里，我们定量地比较了在拟南芥突变体中的三甲基化在H3 K27剥夺了各种关键的PRC组件的变化。我们发现，卷曲叶（CLF），PRC 2的主要催化亚基，协调与不同成员的PRC 1在不同的植物发育程序的抑制。我们发现花发育基因的表达在幼苗中优先通过CLF的非冗余作用被抑制，CLF与LHP 1特异性相关。相反，胚胎发育基因的表达被PRC 1-催化核心亚基AtBMI 1和AtRING 1抑制，与PRC 2-催化酶CLF或SWINGER（SWN）相同。CLF的这种上下文依赖性作用与H3 K27 me 3谱的变化很好地对应，并且与转录因子（TF）的结合基序（包括MADS盒和ABA相关因子）的差异共占据显著相关。我们认为，不同的PRC成员的组合，有区别地调节不同的发展计划，其目标的特异性调制特定的TF。
Polycomb repressive complexes (PRCs) play crucial roles in transcriptional repression and developmental regulation in both plants and animals. In plants, depletion of different members of PRCs causes both overlapping and unique phenotypic defects. However, the underlying molecular mechanism determining the target specificity and functional diversity is not sufficiently characterized. Here, we quantitatively compared changes of tri-methylation at H3K27 in Arabidopsis mutants deprived of various key PRC components. We show that CURLY LEAF (CLF), a major catalytic subunit of PRC2, coordinates with different members of PRC1 in suppression of distinct plant developmental programs. We found that expression of flower development genes is repressed in seedlings preferentially via non-redundant role of CLF, which specifically associated with LIKE HETEROCHROMATIN PROTEIN1 (LHP1). In contrast, expression of embryo development genes is repressed by PRC1-catalytic core subunits AtBMI1 and AtRING1 in common with PRC2-catalytic enzymes CLF or SWINGER (SWN). This context-dependent role of CLF corresponds well with the change in H3K27me3 profiles, and is remarkably associated with differential co-occupancy of binding motifs of transcription factors (TFs), including MADS box and ABA-related factors. We propose that different combinations of PRC members distinctively regulate different developmental programs, and their target specificity is modulated by specific TFs.