Aggregatilinea lenta gen. nov., sp. nov., a slow-growing, facultatively anaerobic bacterium isolated from subseafloor sediment, and proposal of the new order Aggregatilineales ord. nov. within the class Anaerolineae of the phylum Chloroflexi
Aggregatilinea lenta gen. nov., sp. nov., a slow-growing, facultatively anaerobic bacterium isolated from subseafloor sediment, and proposal of the new order Aggregatilineales ord. nov. within the class Anaerolineae of the phylum Chloroflexi
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DOI：
10.1099/ijsem.0.003291
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发表时间：
2019-04-01
影响因子：
2.8
通讯作者：
Imachi, Hiroyuki
中科院分区：
文献类型：
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作者：
Nakahara, Nozomi;Nobu, Masaru K.;Imachi, Hiroyuki
一种新型生长缓慢的兼性厌氧丝状细菌MO-CFX2(T)从日本下田半岛海底沉积物中建立的连续流生物反应器中的产甲烷微生物群落中分离出来。细胞呈多细胞丝状，无运动，革兰氏染色阴性。花丝一般长20多亩（最多约200亩），宽0.5-0.6亩。细胞表面具有毛状结构，细胞质具有多层结构。菌株在20 ~ 37℃（最适30℃）、pH 5.5 ~ 8.0 （pH 6.5 ~ 7.0）、0 ~ 30 g l(-1) NaCl （5 g l(-1) NaCl）条件下生长。在最佳生长条件下，加倍时间和最大细胞密度估计分别为19天左右和10 5个细胞ml（-1）。菌株MO-CFX2(T)在厌氧条件下在有限范围的有机基质上进行化学有机营养生长。细胞脂肪酸主要为饱和脂肪酸C-16:0（47.9%）和C-18:0(36.9%)，不饱和脂肪酸C-18:1 omega 9c（6.0%）和c - 16:1 omega 7（5.1%）。基因组DNA中G+C含量为63.2 mol%。基于16S rRNA基因的系统发育分析表明，菌株MO-CFX2(T)与其最近的亲缘种Thermanaerothrix daxensis GNS-1(T)和thermoarinilinea lacunifontana SW7(T)序列同源性较低（均为58.8%）。基于这些表型和基因组特性，我们建议将MO-CFX2(T) （=KCTC 15625（T), =JCM 32065(T)）命名为Aggregatilinea lenta gen. nov., sp. nov.）。此外，我们还提出了相关的科和目为aggregatilinaceae fam。11月和11月分别为Aggregatilineales。
A novel slow-growing, facultatively anaerobic, filamentous bacterium, strain MO-CFX2(T), was isolated from a methanogenic microbial community in a continuous-flow bioreactor that was established from subseafloor sediment collected off the Shimokita Peninsula of Japan. Cells were multicellular filamentous, non-motile and Gram-stain-negative. The filaments were generally more than 20 mu m (up to approximately 200 mu m) long and 0.5-0.6 mu m wide. Cells possessed pili-like structures on the cell surface and a multilayer structure in the cytoplasm. Growth of the strain was observed at 20-37 degrees C (optimum, 30 degrees C), pH 5.5-8.0 (pH 6.5-7.0), and 0-30 g l(-1) NaCl (5 g l(-1) NaCl). Under optimum growth conditions, doubling time and maximum cell density were estimated to be approximately 19 days and similar to 10 5 cells ml(-1), respectively. Strain MO-CFX2(T) grew chemoorganotrophically on a limited range of organic substrates in anaerobic conditions. The major cellular fatty acids were saturated C-16:0 (47.9 %) and C-18:0 (36.9 %), and unsaturated C-18:1 omega 9c (6.0 %) and C-16: 1 omega 7 (5.1 %). The G+C content of genomic DNA was 63.2 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain MO-CFX2(T) shares a notably low sequence identity with its closest relatives, which were Thermanaerothrix daxensis GNS-1( )(T)and Thermomarinilinea lacunifontana SW7(T) (both 85.8 % sequence identity). Based on these phenotypic and genomic properties, we propose the name Aggregatilinea lenta gen. nov., sp. nov. for strain MO-CFX2(T) (=KCTC 15625(T), =JCM 32065(T)). In addition, we also propose the associated family and order as Aggregatilineaceae fam. nov. and Aggregatilineales ord. nov., respectively.