Normal pre-B cells express a receptor complex of mu heavy chains and surrogate light-chain proteins.
Normal pre-B cells express a receptor complex of mu heavy chains and surrogate light-chain proteins.
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DOI：
10.1073/pnas.88.14.6284
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发表时间：
1991-07
影响因子：
11.1
通讯作者：
N. Nishimoto;H. Kubagawa;T. Ohno;G. Gartland;A. Stanković;Max D. CooPE
中科院分区：
文献类型：
--
作者：
N. Nishimoto;H. Kubagawa;T. Ohno;G. Gartland;A. Stanković;Max D. CooPE
B细胞的前体细胞构成骨髓中淋巴细胞的亚群，可通过其表面非免疫球蛋白标记物的表达和免疫球蛋白κ和λ轻链的缺失来鉴定。大多数前B细胞合成μ重链，但没有轻链伴侣，这些经历快速的细胞质降解。在本研究中，我们证明，晚期前B细胞，像他们的肿瘤对应物，表达低水平的表面受体组成的μ链配对的替代轻链复合物形成的Vpre-B和λ 5样蛋白。这些数据定义了一个以前怀疑但未被识别的正常前B细胞分化阶段。克隆多样性受体的表达使得该未成熟B系细胞群体可能容易受到抗原和独特型相互作用的克隆选择。
Precursors of B cells, which constitute a subpopulation of the lymphocytes in bone marrow, can be identified by their surface expression of nonimmunoglobulin markers and the absence of immunoglobulin kappa and lambda light chains. Most pre-B cells synthesize mu heavy chains but, without light-chain partners, these undergo rapid cytoplasmic degradation. In the present study, we demonstrate that late stage pre-B cells, like their neoplastic counterparts, express low levels of a surface receptor composed of mu chains paired with a surrogate light-chain complex formed by Vpre-B and lambda 5-like proteins. The data define a previously suspected but unrecognized stage in normal pre-B-cell differentiation. Expression of a clonally diverse receptor renders this population of immature B-lineage cells potentially vulnerable to clonal selection by antigens and idiotypic interactions.