Improved bone-forming functionality on diameter-controlled TiO2 nanotube surface
Improved bone-forming functionality on diameter-controlled TiO2 nanotube surface
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DOI：
10.1016/j.actbio.2009.05.008
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发表时间：
2009-10-01
影响因子：
9.7
通讯作者：
Jin, Sungho
中科院分区：
文献类型：
--
作者：
Brammer, Karla S.;Oh, Seunghan;Jin, Sungho
二氧化钛（TiO 2）纳米管表面能够显著加速成骨细胞粘附，并与骨形成牢固的结合。我们通过阳极氧化在钛基底上制备了不同尺寸（直径30-100 nm）的二氧化钛（TiO 2）纳米管，并研究了成骨细胞对这些不同纳米管尺寸的响应行为。这项研究的独特和引人注目的结果是，在相对狭窄的纳米管尺寸范围内获得了成骨细胞行为的变化，小直径（类似于30 nm）的纳米管促进了成骨细胞粘附的最高程度，而较大直径（70-100 nm）的纳米管引起了具有极其细长的细胞形态和高得多的碱性磷酸酶水平的较低的细胞群体。也观察到更大直径的纳米管的核的伸长增加。通过控制纳米形貌，大直径的纳米管，在类似于100分钟的制度，诱导极其细长的细胞形状，具有11：1的纵横比，这导致碱性磷酸酶活性的大幅增强的上调，这表明更大的骨形成能力比直径较小的纳米管。这种纳米管结构已经是一种强骨整合植入材料，为开发和优化新型骨科相关治疗提供了令人鼓舞的意义，并精确控制了所需的细胞和骨生长行为。(C)2009 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The titanium dioxide (TiO2) nanotube surface enables significantly accelerated osteoblast adhesion and exhibits strong bonding with bone. We prepared various sizes (30-100 nm diameter) of titanium dioxide (TiO2) nanotubes on titanium substrates by anodization and investigated the osteoblast cellular behavior in response to these different nanotube sizes. The unique and striking result of this study is that a change in osteoblast behavior is obtained in a relatively narrow range of nanotube dimensions, with small diameter (similar to 30 nm) nanotubes promoting the highest degree of osteoblast adhesion, while larger diameter (70-100 nm) nanotubes elicit a lower population of cells with extremely elongated cellular morphology and much higher alkaline phosphatase levels. Increased elongation of nuclei was also observed with larger diameter nanotubes. By controlling the nanotopography, large diameter nanotubes, in the similar to 100 min regime, induced extremely elongated cellular shapes, with an aspect ratio of 11:1, which resulted in substantially enhanced up-regulation of alkaline phosphatase activity, suggesting greater bone-forming ability than nanotubes with smaller diameters. Such nanotube structures, already being a strongly osseointegrating implant material, offer encouraging implications for the development and optimization of novel orthopedics-related treatments with precise control toward desired cell and bone growth behavior. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.