Virtual terminator nucleotides for next-generation DNA sequencing.
Virtual terminator nucleotides for next-generation DNA sequencing.
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DOI：
10.1038/nmeth.1354
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发表时间：
2009-08
期刊：
影响因子：
48
通讯作者：
Thompson, John F.
中科院分区：
文献类型：
--
作者：
Bowers, Jayson;Mitchell, Judith;Beer, Eric;Buzby, Philip R.;Causey, Marie;Efcavitch, J. William;Jarosz, Mirna;Krzymanska-Olejnik, Edyta;Kung, Li;Lipson, Doron;Lowman, Geoffrey M.;Marappan, Subramanian;McInerney, Peter;Platt, Adam;Roy, Atanu;Siddiqi, Suhaib M.;Steinmann, Kathleen;Thompson, John F.
我们合成了用于下一代测序的带有拴系抑制物的可逆终止子。它们被高效地高保真地掺入，同时防止了额外的核苷酸掺入，并被用于在单分子系统中对犬细菌人工染色体进行测序，该系统提供了超过99%的测序区域的均匀复盖率。这种单分子方法产生了高质量的序列数据，而不需要目标扩增，从而避免了伴随的偏差。
We synthesized reversible terminators with tethered inhibitors for next generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides and were used to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high quality sequence data without the need for target amplification and thus avoided concomitant biases.
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影响因子：
14.9
作者：
Wu, Weidong;Stupi, Brian P.;Metzker, Michael L.
通讯作者：
Metzker, Michael L.
影响因子：
2.9
作者：
KUCHTA, RD;MIZRAHI, V;BENKOVIC, SJ
通讯作者：
BENKOVIC, SJ
影响因子：
64.8
作者：
Bentley DR;Balasubramanian S;Swerdlow HP;Smith GP;Milton J;Brown CG;Hall KP;Evers DJ;Barnes CL;Bignell HR;Boutell JM;Bryant J;Carter RJ;Keira Cheetham R;Cox AJ;Ellis DJ;Flatbush MR;Gormley NA;Humphray SJ;Irving LJ;Karbelashvili MS;Kirk SM;Li H;Liu X;Maisinger KS;Murray LJ;Obradovic B;Ost T;Parkinson ML;Pratt MR;Rasolonjatovo IM;Reed MT;Rigatti R;Rodighiero C;Ross MT;Sabot A;Sankar SV;Scally A;Schroth GP;Smith ME;Smith VP;Spiridou A;Torrance PE;Tzonev SS;Vermaas EH;Walter K;Wu X;Zhang L;Alam MD;Anastasi C;Aniebo IC;Bailey DM;Bancarz IR;Banerjee S;Barbour SG;Baybayan PA;Benoit VA;Benson KF;Bevis C;Black PJ;Boodhun A;Brennan JS;Bridgham JA;Brown RC;Brown AA;Buermann DH;Bundu AA;Burrows JC;Carter NP;Castillo N;Chiara E Catenazzi M;Chang S;Neil Cooley R;Crake NR;Dada OO;Diakoumakos KD;Dominguez-Fernandez B;Earnshaw DJ;Egbujor UC;Elmore DW;Etchin SS;Ewan MR;Fedurco M;Fraser LJ;Fuentes Fajardo KV;Scott Furey W;George D;Gietzen KJ;Goddard CP;Golda GS;Granieri PA;Green DE;Gustafson DL;Hansen NF;Harnish K;Haudenschild CD;Heyer NI;Hims MM;Ho JT;Horgan AM;Hoschler K;Hurwitz S;Ivanov DV;Johnson MQ;James T;Huw Jones TA;Kang GD;Kerelska TH;Kersey AD;Khrebtukova I;Kindwall AP;Kingsbury Z;Kokko-Gonzales PI;Kumar A;Laurent MA;Lawley CT;Lee SE;Lee X;Liao AK;Loch JA;Lok M;Luo S;Mammen RM;Martin JW;McCauley PG;McNitt P;Mehta P;Moon KW;Mullens JW;Newington T;Ning Z;Ling Ng B;Novo SM;O'Neill MJ;Osborne MA;Osnowski A;Ostadan O;Paraschos LL;Pickering L;Pike AC;Pike AC;Chris Pinkard D;Pliskin DP;Podhasky J;Quijano VJ;Raczy C;Rae VH;Rawlings SR;Chiva Rodriguez A;Roe PM;Rogers J;Rogert Bacigalupo MC;Romanov N;Romieu A;Roth RK;Rourke NJ;Ruediger ST;Rusman E;Sanches-Kuiper RM;Schenker MR;Seoane JM;Shaw RJ;Shiver MK;Short SW;Sizto NL;Sluis JP;Smith MA;Ernest Sohna Sohna J;Spence EJ;Stevens K;Sutton N;Szajkowski L;Tregidgo CL;Turcatti G;Vandevondele S;Verhovsky Y;Virk SM;Wakelin S;Walcott GC;Wang J;Worsley GJ;Yan J;Yau L;Zuerlein M;Rogers J;Mullikin JC;Hurles ME;McCooke NJ;West JS;Oaks FL;Lundberg PL;Klenerman D;Durbin R;Smith AJ
通讯作者：
Smith AJ
DOI：
10.1073/pnas.0230489100
发表时间：
2003-04-01
影响因子：
11.1
作者：
Braslavsky, I;Hebert, B;Quake, SR
通讯作者：
Quake, SR
DOI：
10.1073/pnas.0609513103
发表时间：
2006-12-26
影响因子：
11.1
作者：
Ju, Jingyue;Kim, Dae Hyun;Turro, Nicholas J.
通讯作者：
Turro, Nicholas J.