Purification and characterization of a pheromone that induces sexual cell division in the unicellular green alga Closterium ehrenbergii
Purification and characterization of a pheromone that induces sexual cell division in the unicellular green alga Closterium ehrenbergii
复制标题
诱导单细胞绿藻新月藻有性细胞分裂的信息素的纯化和表征
DOI：
10.1016/s0981-9428(01)01356-0
复制
发表时间：
2002
影响因子：
6.5
通讯作者：
H. Sekimoto
中科院分区：
文献类型：
--
作者：
R. Fukumoto;N. Dohmae;K. Takio;S. Satoh;T. Fujii;H. Sekimoto
新月藻是一种单细胞轮藻，由两种性别组成：交配型正（mt+）和负（mt-）。有性生殖过程包括五个步骤：有性对的形成，一对中每个成员的细胞分裂，接合乳头的形成，配子囊细胞释放原生质体，以及原生质体融合形成合子。第二步，称为性细胞分裂（SCD），从一个营养母细胞产生两个配子囊细胞。mt ~+细胞的SCD是由一种可扩散的性信息素介导的，称为SCD诱导信息素（SCD-IP）。这种信息素在光照下从mt细胞中释放出来，mt+细胞的存在刺激mt细胞分泌信息素。SCD-IP通过连续柱色谱分离从共培养两种交配型细胞的培养基中纯化。纯化的SCD-IP是一种表观分子量为20 kDa的糖蛋白。通过质谱法估计SCD-IP的分子量为18 kDa。该信息素的氨基末端和两个内部氨基酸序列与另一种新月藻信息素---新月藻-糙伏毛-滨海新月藻复合体（C. peracerosum-strigosum-littorale complex）的原生质体释放诱导蛋白（PR-IP）诱导剂（C. peracerosum-strigosum-littorale complex）具有相似性。pslc）。这两种信息素诱导不同的形态反应，在每个新月藻种。在此基础上，对性信息素的多样性进行了讨论。
Closterium ehrenbergii is a unicellular charophycean alga consisting of two sexes: mating type plus (mt+) and minus (mt–). The sexual reproductive process consists of five steps: formation of sexual pairs, cell division of each member of a pair, formation of conjugation papillae, release of protoplasts from gametangial cells, and fusion of protoplasts to form a zygote. The second step, called sexual cell division (SCD), produces two gametangial cells from one vegetative mother cell. The SCD of mt+cell is mediated by a diffusible sex pheromone, named SCD-inducing pheromone (SCD-IP). This pheromone is released from mt–cells in the light, and the presence of mt+cells stimulates its secretion from mt–cells. SCD-IP was purified by sequential column-chromatographic fractionation from culture medium in which both mating type cells had been co-cultured. Purified SCD-IP is a glycoprotein with an apparent molecular mass of 20 kDa. The molecular mass of the SCD-IP was estimated to be 18 kDa by mass spectrometry. Amino-terminal and two internal amino acid sequences of the pheromone revealed significant similarity to another Closterium pheromone, protoplast release-inducing protein (PR-IP) inducer of Closterium peracerosum-strigosum-littorale complex (C. pslc). These two pheromones induced different morphological reactions in each Closterium species. Based on these results, the diversity of sex pheromones is discussed.
Closterium peracerosum-strigosum-littorale 复合体中性信息素的不对称生殖隔离
DOI：
--
发表时间：
2008
期刊：
影响因子：
--
作者：
Tsuchikane;Y.;Yamazaki;K.;Ito;M.;Sekimoto;H
通讯作者：
H